Essential oils of medicinal plants have been used traditionally against pathogenic bacteria that caused infectious disease in human and microbial spoilage of food and have been used safely in herbal medicine as antibacterial compounds. In the present study, the antibacterial activities of the oils were evaluated against human and animals pathogenic bacteria. In this assay, the selective plants reported ethnobotanical uses traditionally and also were referenced in some herbal medicine text. The essential oil of Stachys pubescens, Mentha piperita, Clinopodium vulgare and Satureja hortensis were prepared by hydrodistillation and were analyzed by gas chromatography/mass spectrometry (GC/MS). The number 23, 22, 21 and 21 components were identified in S. pubescens, S. hortensis, M. piperita and C. vulgare, respectively. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of oils were determined with broth microdilution and agar diffusion method on bacterial strains. Results from the antibacterial testing indicated that S. pubescens, M. piperita and C. vulgare essential oils showed high activities and inhibited the growth of all the selected bacteria. While the essential oil of S. hortensis displayed the moderate potential activity. Our finding supported the notion that plant essential oils composition or total extract may have a role as pharmaceuticals and preservatives effects as safely and effective drugs with low resistance against microorganisms. Therefore, these essential oils could be used for management of these pathogens as a potential source of sustainable eco-friendly botanical bactericides.