Background: Periodontal dressing commonly used in dentistry today does not contain compounds that can accelerate wound healing. Lime (Citrus aurantifolia Swingle) peel contains flavonoids that play a role in increasing fibroblast cells so that they can accelerate the healing process. Periodontal dressings supplemented with lime (Citrus aurantifolia Swingle) peel extract are expected to provide an alternative material that can accelerate wound healing in addition to closure. Purpose: The study aims to determine the effect of adding lime (Citrus aurantifolia Swingle) peel extract to periodontal dressings on the increase in the number of fibroblasts in the gingival healing process. Methods: The study was conducted in an experimental laboratory in vivo. The study used a post-randomised control group of 32 rabbits with lesions of the mandibular gingiva using a 2 mm diameter punch biopsy. The experimental animals were divided into 8 groups, namely the control group, which was treated with periodontal dressings without the addition of lime (Citrus aurantifolia Swingle) peel extract, and the treatment group, which was treated with periodontal dressings with the addition of the extract. Histological observations of the tissues were performed with HE staining to count the number of fibroblasts. Results: Statistical test results showed that there was a significant difference in the number of fibroblasts between the control group and the treatment group on day 3 and day 5 (ANOVA, p <0.05). Conclusion: Adding extra lime (Citrus aurantifolia Swingle) peel to the periodontal dressing increases the number of fibroblast cells after gum injury.

Periodontitis is inflammation of the periodontal tissue that can cause progressive damage to the periodontal ligament and alveolar bone. Clinical examination to determine the degree of damage to the alveolar bone using a periodontal probe instrument or probing. Cellular activity in the gingival crevicular fluid is thought to be a biomarker to determine the inflammatory status of the periodontal tissue. Cellular immune responses suggest the role of cytokines in the pathogenesis of gingivitis and periodontitis. The cytokines observed in this study were the protein Osteocalcin and Interleukin-1β (IL-1β) in the gingival crevicular fluid (GCF). In this study, 67 patients were examined for the condition of their periodontal tissues including the severity of gingivitis and periodontal probing. The gingivitis group was divided into 4 groups, namely normal, mild, moderate, and severe. Meanwhile, for probing samples were grouped based on probing into 3 groups. Group I (0-3 mm), group II (3.1-5 mm) and group III (> 5 mm). GCF was obtained using paper points that were left in the gingival sulcus for 30 seconds. Then paper points are put into cryovial tubes which are sent to the laboratory for ELISA test. The observed data on the expression of Osteocalcin and IL-1β were then performed statistical tests. Osteocalcin and IL-1β expression showed a significant difference with the severity of gingivitis cases. In the other hand, it did not show a significant difference with difference of periodontal pocket depth. Osteocalcin and IL-1β expression associated with the severity of gingivitis cases.