Background: A sealer is required during obturation to fill space between gutta-percha and canal walls. It provides an impervious seal, fills the irregularities and minor discrepancies between the root canal wall and core filling material, and assists in microbial control. Aim: To evaluate the penetration depth and percentage area of a root canal sealer placed by five different techniques using confocal laser scanning microscope (CLSM). In our study we compared ultrasonic, endoactivator (sonic device), lentulospiral (rotary) and master gutta-percha with NiTi spreader in reciprocating hand piece for sealer placement using AH Plus sealer. Methods: Thirty extracted then decoronated and standardised mandibular premolars were prepared by Protaper rotary files up to master apical file F3. Samples were randomly divided into five groups for sealer placement; Group 1, Ultrasonic file; Group 2, Endoactivator; Group 3, Reciprocating hand piece with NiTi spreader; Group 4, Rotary Lentulospiral; Group 5, Master apical gutta-percha .Two root sections from apical and coronal third were analysed for sealer distribution and depth of sealer penetration by confocal microscope, using the ruler tool of the IOB software (Olympus). Results: Results showed that maximum depth and percentage of sealer penetration is shown by Group 1; ultrasonic file and least by Group 5; master apical gutta-percha both at apical as well as coronal levels. Conclusion: Sealer penetration is influenced by placement method and new method of using reciprocating NiTi spreader was comparable to ultrasonic method which showed best sealer penetration and distribution.