The present study was carried out to evaluate the antimicrobial properties of two traditional medicinal plant species Saponaria officinalis and Zanthoxylum aramatum belonging to the families Raryophyllaceae and Rutaceae. The root part was selected for the antibacterial activity, and extracts were prepared using soxhlet extraction procedure using different solvents successively. Different extracts of selected plants tested their anti-microbial property using agar well diffusion method at different concentration on standard human pathogenic bacterial strains. The selected plants extracts concentrated dependent bactericidal activity on tested standard bacterial strains. The zones of inhibitions were measured for each concentration of various extracts on each bacterial strain tested in a millimeter (mm). All concentrations of extracts showed minimum zone of inhibition (8mm). The chloroform extracts at 5mg/100µL showed less activity. But, as the concentration increases the bactericidal activity was increased. The extracts showed more activity at 40mg/100µL. Among two plants S. officinalis showed more antibacterial activity. In these, methanol extracts of two plants more competent results along with ciprofloxacin on tested bacterial strains. The extracts also showed more activity on gram-negativeorganisms compared to gram-positiveorganisms. The extracts showed more activity on E. coli and S. typhimurium and lower activity on C. sporogenes and S. pneumoniae. The results of the present study provide the evidence on antibacterial property of selected medicinal plants and there is a scope to further studies on isolation of antimicrobial compounds from these species.

Background: The using of synthetic drugs in adequate manner for the treatments of liver diseases are causes the different side effect. Therefore, there is need to search for new bioactive compounds to control liver diseases. In this point of view, we selected the medicinal plant H. mystax to evaluate of its antioxidant and hepatoprotective potentiality and isolation of its constituents, because it has been using in traditional medicine in the treatment of different diseases. Methods: Different extracts were prepared from H. mystax using maceration extraction process.Antioxidant activity was carried out using standard test procedures on Superoxide, Hydroxyl and DPPH free radicals. Hepatoprotective was tested using Thioacetamide (TAA) induced liver intoxication in rats. Results: The H. mystax extracts were showed dose dependent antioxidant and hepatoprotective activities. Tested extracts of H. mystax have more activity on hydroxyl free radical than superoxide and DPPH radicals and among all extracts hydroalcoholic extract showed more activity. The extracts of H. mystax significantly restored the altered biochemical parameters due to TAA induced liver intoxication and hydroalcoholic extract exhibited more significant activity. So, the hydroalcoholic extract was used for the isolation of phytochemical constituents through column chromatography and isolated HM-1 and HM-2 compounds and are identified as stigmasterol and β-sitosterol. Conclusion:From the results of the study, it could be conclude that H. mystax have the capacity in reduction of free radicals and normalization of liver function when it damage due to different liver intoxications and further research is need to evaluate more pharmacological activities and in isolation of most active bioactive compounds from H. mystax.