Introduction and Aim: Glaucoma is a chronic optic neuropathy which is the leading cause of irreversible blindness across the globe. It is known that Intraocular pressure is responsible for these altered changes but recent experimental and clinical studies have reported that cerebrospinal fluid pressure (CSFP) and trans-lamina cribrosa pressure difference (TLCPD), may be an important cause of glaucomatous optic nerve damage, especially in subjects with normal-tension glaucoma. To assess the correlation between ocular perfusion pressure (OPP) and TLCPD in healthy young adults. Materials and Methods: One hundred healthy young adult volunteers comprising of 50 obese (25 males and 25 females) and 50 non-obese (25 males and 25 females) in the age group of 18-19 years among MBBS Phase I students of JSS Medical College, JSSU, Mysore were the subjects for the study. Blood Pressure and Intra Ocular Pressure were recorded. Mean arterial pressure (MAP), OPP, CSFPand TLCPD were calculated. Results: There exists a positive correlation (0.50) between OPP and TLCPD. There was a statistically significant difference in OPP, CSFP and TLCPD between obese and non-obese groups. Conclusion: The present study provides information on the relationship of TLCPD with CSFP and OPP that are higher in obese individuals.