Objective: The present study was carried out to estimate the possible role of Interleukin-4 (IL-4)RαQ576R genes polymorphism in the development of immune reaction against penicillin, as well as to study the effect of IL-4 cytokine in regulating allergic reactions.Materials and Methods: Measurement of serum IL-4 concentration was done using enzyme-linked immunosorbent assay technique; IL-4RαQ576R gene polymorphisms were genotyped using polymerase chain reaction-restriction fragment lengths polymorphisms. Comparisons for statistical significance were performed using Mann–Whitney U-test.Results: Comparing with control subjects, there was a significantly increased level of IL-4 (348.53 pg/ml) in penicillin allergic patients versus (284.72 pg/ml) in sera of control subjects. The IL-4RαQ576R alleles were significantly higher in the penicillin allergic individual compared with apparently healthy control subjects.Conclusions: Data study suggested that IL-4 cytokine have some important roles in penicillin hypersensitivity reaction, additionally the IL- 4RαQ576Rgene polymorphisms might involve in modulating of penicillin hypersensitivity.

Background: Neonatal sepsis (NS) is a clinical syndrome of systemic

Psoriasis is an autoimmune inflammatory disease of human skin with the etiology being unknown and for which there is no cure. It is believed to be genetically and immunologically conditioned and has major negative impact on quality of life. This study aimed to determine the impact of inheritance of specific human leukocyte antigen-C loci and some sociodemographic factors on the susceptibility to early onset psoriasis (type I). The current study included psoriatic group involving 76 patients (type I) and a match of apparently healthy group comprising 87 persons as a control. A polymerase chain reaction based method (low resolution sequence specific primer) was used to detect C*06, C*07 and C*17 allele after informed consent. The study showed that the C*06 and C*07 allele were significantly associated with early onset psoriasis (p-value < 0.05), while C*17 showed no significant association. There was also a higher percentage of patients in urban districts (84.2%) than rural residents (15.8%). There was no significant association between smoking and type I psoriasis (p-value > 0.05). Both of C*06 and C*07 genotypes increased the risk of early onset psoriasis, while rural residency decreased the chance of getting type I psoriasis. Furthermore, the lack of association with smoking could not mitigate the effect of passive smoking.

Background: Schizophrenia is a severe mental disorder that affects

Objective Identify the association between single nucleotide polymorphisms (SNP) of IL-1 Beta and the onset and severity of the disease. Pre-eclampsia (PE) is a relatively common, systemic pregnancy disorder characterized by the development of concurrent hypertension (>140/90 mmHg) and proteinuria (>300 mg/24 h) at ≥20 weeks of gestation, that may also be associated with a myriad of other symptoms such as edema, headache, blurred vision, irritability, abdominal pain, and thrombocytopenia. Methods The extracted DNA was amplified for IL-1 Beta RFLP in 60 clinically diagnosed preeclampsia pregnant women and 60 normotensive pregnant women. For statistical significance, OR was measured and all data were processed by using SPSS. Results IL-1 Beta genotyping in PE pregnant women showed the following results TT genotype having 2.33 fold risk of having PE and etiological factor (0.57) while TC mutant genotype showed 1.125 fold risk of having PE etiological factor of (0.111), while homozygous CC genotype considered as protective genotype with protective factor of (0.674). T allele considered the etiological factor of (0.287) while C allele considered as protective allele with protective factor of (0.589). Conclusion IL-1 Beta T allele considered as significant risk factor for having preeclampsia. The presence of IL-1 Beta C alleles protects against having preeclampsia.