The hospital environment, medical instruments and health care staff considered as a reservoir of potential pathogens that colonized by microorganisms which can cause infections. Bacteria could be survived up to months on dry inanimate surfaces and could be transmitted to the patients or visitors through the hand when contact with contaminated medical devices.The study was achieved from June 2017 to December 2019, at the most three occupied departments at Teaching Hospital in Baghdad. Swabs were collected from medical instruments and surfaces. Samples were cultured and incubated for bacterial and fungal pathogens respectively, through the standard microbiological procedures.From a total of 1043 swab, the highest samples were collected in the year of 2018(455) swab, as well as, the highest percentages of bacteria were isolated. Mixed bacterial growth was isolated from 82 swabs (7.86 %), while 961 swabs (92.13%) did not show any growth. the isolates had 54 (65.85%) Gram-positive bacteria, which were Bacillus then Staphylococcus epidermidis (28 and 11) isolates respectively, while 28 (34.14%) were Gram-negatives, that consisted of Pseudomonas aeruginosa 13 isolates, followed by Escherichia coli 11 isolates. The Gynecology Department carries higher rates of bacterial contamination (50%) and the Ultrasound units in both of (Gynecology and Surgery) Departments revealed a high percentage of contamination (24.39%, 29.16%) respectively.In conclusion, the contamination rates with Gram-positive bacteria increased even after regular cleaning and disinfection in the environment surrounding patients and medical devices in the hospital.

Background:The hospital environment, the design of buildings especially operating theaters and associated facilities play an important role in the spread of nosocomial infections and surgical site infection.Methods: A descriptive study. It covers 30 months; Weekly swabs were collected from thirty-two operating theaters. Results:The highest rate of contamination was in the general surgical OT with 12.9%, followed by Orthopedic OT (10.7%). On the other hand, Ophthalmic OT recorded the least bacterial contamination with (3.1%) only.During the three years' time of the study; we saw heavy growth of G-ve bacteria:P.aurogenosa195(35.8%), 137(43.4%) and 48(53.3%), thenE. coli141(25.9%), 115(%) and 28(%) respectively. Conclusion:Contamination was identified in all OT in different branches. Bacterial growth reported throughout the year and in different months. Despite the availability and usage of the sterilization materials, contamination still there.

Objective: To evaluate the level of contamination in different hospital's wards and kitchens in AL-Yarmouk teaching hospital in Baghdad -Iraq. Method:The study was done from the 1 st of January, 2017 to the 30 th of June, 2019. A total of 7742sterile cotton swabs were collected randomly from the following wards: Burns, RCU, CCU, NICU, (Dialysis, Bronchoscopy, Endoscopy) unites and Kitchens. Samples were cultured in different agars and incubated for 24-48 hrs at 25 °C and 37 °C for fungi and bacterial growth, respectively.Results: Out of 7742 sample, 1227 swabs were reported positive. By 2017, Gram-positive bacteria were more prevalent with 59.5%, and Bacillus was the most common isolated .The rates of Gram-negative bacteria became more predominant with 54.9% and 69.4% in 2018 and 2019, respectively and E. coli was the main isolated bacteria with prevalence rates of 32.1% in (2018) and 42.4% in (2019). The most contaminated wards were