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The comprehend assess the pharmacognostic characteristics of an important ethnomedicinal plant, O. quadripartita Salz. ex Decne. The pharmacognostic evaluation was executein terms of taxonomic, macroscopic, microscopic, fluorescence analysis, and phytochemical analysis. The Osyaris members heartwood majorly used to make furniture and perfumes. The Osyris plant members are known for fragrance and volatile oil constituents. The microscopic features of stem bark were observed as cork, cortex, phloem fibers, phloem medullary rays, and scleridesin the transverse section (T.S.) and longitudinal section (L.S.). The microscopy of stem bark powder observed the presence of cortex cells, sieve tubes, calcium oxalate crystals, and lignified fibers. The initial screening of phytochemical constituents of stem bark crude plant extracts indicate the presence of carbohydrates, proteins, alkaloids, glycosides, flavonoids, saponins, tannins & phenolic in different polar and non-polar solvent systems such as petroleum ether, chloroform, acetone, methanol, and water. Many pharmacognostic characters observed during the study, it's help in the standardization of O. quadripartite.