Identification of resistant germplasms is crucial for crop breeding. The aim of this study was to determine the reaction of accessions of watermelon plants to Meloidogine enterolobii using two experiments. In the first experiment, 20 accessions were evaluated, and in the second, the four most promising accessions were selected in the first experiment and two controls. Both experiments were implemented under a completely randomized design with ten replications. Each parcel consisted of a plastic pot containing autoclaved soil inoculated with 2,200 eggs of M. enterolobii. Among the variables studied, the egg number and reproduction factor showed a high positive correlation. The subsamples indicated a wide variation among and within each accession (p < 0.01). At the end, variation in the response among and within the accessions was observed regarding the reproduction factor. Of the accessions evaluated, accessions 9 and 10 exhibited potential for the implementation of breeding programs.