No abstract

Ibrutinib is a selective inhibitor of Bruton’s tyrosine kinase, which is used in chronic lymphocytic leukemia treatment. In this study, an adsorptive stripping square wave voltammetry was developed to quantify the nanomolar level of ibrutinib in an anionic surfactant medium. The effect of supporting electrolyte, pH, surfactant concentration, and scan rate on the voltammetric peak current and potential of ibrutinib were evaluated using a glassy carbon electrode. The possible electrochemical mechanism was discussed with model compounds. The electrochemical behavior of ibrutinib shows irreversible and diffusion-adsorption mixed controlled oxidation processes depending on scan rate studies in 0.1 M sulphuric acid. The sensitivity of the method was increased by the combined use of surfactant and stripping conditions. Under optimum conditions, a concentration range of 4.00 x 10-9 to 2.00 x 10-7 M was linear in 0.1 M sulphuric acid, including 2.00 × 10-3 M sodium lauryl sulphate. The limit of detection was calculated as 2.73 x 10-10 M. The proposed method was applied for the quantification of ibrutinib in biological samples. In addition, this method was evaluated in the presence of some potential interference substances.