The capacity for five Brettanomyces bruxellensis strains to form biofilm on stainless steel was confirmed, and the sanitation of these biofilms was tested using a solution of lactic acid and a reference method (a solution of foaming caustic soda and peroxide at 5 %). Different responses were observed depending on the strain: lactic acid solution induced a slight reduction in cell population, while the reference method resulted in the elimination of the adhered cells for three strains, but generated VBNC states for two others. The effects of sanitation on the biofilm formed is strain-dependent.