Background: Sea algae are widely used as food and cosmetics in the world. There are several algae including brown algae which are us for human used to maintain health and skin care. Brown algae have various potential biological activities because contain substantial phytochemical constituent. Numerous report has identified phytochemical compound of Sargassum sp. extract but the activity as anti-collagenase almost none. Objective: To study the antioxidant, and anti-collagenase activity of Sargassum plagyophyllum extract as active pharmaceutical ingredient for anti-wrinkle cosmetics. Methods: Sargassum plagyophyllum obtained from Pasauran Beach, Banten, West Java, Indonesia. The extract Sargassum plagyophyllum extracted with three concentration ethanol-water: (E1) ethanol 25%, (E2) ethanol 50% and (E3) ethanol 75%, by using maceration extraction method for 24 h, thrice. The extract was evaluated include total phenolic content, antioxidant activity, and the best extract was tested for the anti-collagenase activity. Results: Total phenol in the extract were 0.588 ± 0.01 (E1), 0.272 ± 0.01 (E2), and 0.220± 0.03 (E3) mg PGE/ 100 g extract, respectively. Antioxidant activity of the extract (50 mg/mL) was 41,61±0,02% (E1), 39,16±0,01% (E2), 37,58±0,03% (E3) and ascorbic acid 78.03±0,65 % (22.44 µg/mL) as a standard. The best extract (E1) had inhibited the activity of collagenase by 54.46 ± 0.37%. Conclusion: The brown seaweed (Sargassum plagyophyllum) extract can be used as an active pharmaceutical ingredient for anti-wrinkles cosmetic