The identification of new biomarkers is essential in the implementation of personalized health care strategies that offer new therapeutic approaches with optimized and individualized treatment. In support of hypothesis generation and testing in the course of our biomarker research an online portal and respective function-tested reverse transcription quantitative real-time PCR assays (RT-qPCR) facilitated the selection of relevant biomarker genes. We have established workflows applicable for convenient high throughput gene expression analysis in biomarker research with cell lines (in vitro studies) and xenograft mouse models (in vivo studies) as well as formalin-fixed paraffin-embedded tissue (FFPET) sections from various human research and clinical tumor samples. Out of 92 putative biomarker candidate genes selected in silico, 35 were shown to exhibit differential expression in various tumor cell lines. These were further analysed by in vivo xenograft mouse models, which identified 13 candidate genes including potential response prediction biomarkers and a potential pharmacodynamic biomarker. Six of these candidate genes were selected for further evaluation in FFPET samples, where optimized RNA isolation, reverse transcription and qPCR assays provided reliable determination of relative expression levels as precondition for differential gene expression analysis of FFPET samples derived from projected clinical studies. Thus, we successfully applied function tested RT-qPCR assays in our biomarker research for hypothesis generation with in vitro and in vivo models as well as for hypothesis testing with human FFPET samples. Hence, appropriate function-tested RT-qPCR assays are available in biomarker research accompanying the different stages of drug development, starting from target identification up to early clinical development. The workflow presented here supports the identification and validation of new biomarkers and may lead to advances in efforts to achieve the goal of personalized health care.

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In this paper, we introduce a research project on investigating the relation of computers and humans in the field of wearable activity recognition. We use an interdisciplinary approach, combining general philosophical assumptions on the mediating character of technology with the current computer science design practice. Wearable activity recognition is about computer systems which automatically detect human actions. Of special relevance for our research project are applications using wearable activity recognition for self-tracking and self-reflection, for instance by tracking personal activity data like sports. We assume that activity recognition is providing a new perspective on human actions; this perspective is mediated by the recognition process, which includes the recognition models and algorithms chosen by the designer, and the visualization to the user. We analyze this mediating character with two concepts which are both based on phenomenological thoughts namely first Peter-Paul Verbeek's theory on human-technology relations and second the ideas of embodied interaction. Embedded in the concepts is a direction which leads to the role of technical design in how technology mediates. Regarding this direction, we discuss two case studies, both in the possible using practice of self-tracking and the design practice. This paper ends with prospects towards a better design, how the technologies should be designed to support self-reflection in a valuable and responsible way.

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