Natural rubber latex (NRL) is a complex colloidal system composed of rubber particles surrounded by a layer of phospholipids and proteins maintaining its colloidal stability. NRL creaming is a nonrobust concentration method, and its colloidal and rheological properties can be altered due to the presence of creaming agents and surfactants. Both properties must remain stable over time after creaming to ensure conditions for subsequent manufacturing processes.These properties have been evaluated for synthetic latex, but not for NRL in which the clone variety and phenological factors make the system being always different. In this research, hydroxyethyl cellulose (HEC) and sodium dodecyl sulphate (SDS) are used to concentrate NRL and response surface methodology was selected to optimize the colloidal and rheological properties of creamed NRL. Flocculation and stabilization by depletion are involved on NRL concentration depending on HEC and SDS concentration. Those agents modify NRL zeta potential in a range of À78 and À63 mV, and viscosity increases more than 10 times the raw NRL value. SDS plays a key role on enhancement of the creaming process and in electrostatic stability because its anionic nature. Physicochemical interactions between NRL, HEC, and SDS are proposed.