Objective. The aim of the study is to explore the relationship between matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9) gene polymorphisms and the risk of colorectal cancer. Methods. From January 2019 to December 2021, 308 patients with colorectal cancer in our hospital were selected to be included in the colorectal cancer group and 300 normal healthy people were included in the control group. We perform genotyping, compare the genotype frequencies between the colorectal cancer group and the control group, calculate the relationship between MMP-2 and MMP-9 gene polymorphisms and disease risk, and analyze the genotype distribution characteristics of colorectal cancer patients with different pathological stages and lymph node metastasis status. The expression levels of serum MMP-2 and MMP-9 in patients with different genotypes were compared. Results. The frequency of CC genotype and C gene at the MMP-2 gene−735 (C/T) locus in the colorectal cancer group was higher than that of the control group, and the frequency of TT genotype and T gene at MMP-9 gene−1562 (C/T) locus was a higher control group ( P < 0.05 ). The comparison of genotype and gene frequency distribution of MMP-2 gene−1306 (C/T), −790 (T/G), and MMP-9 gene R668Q and P574R between the colorectal cancer group and the control group ( P > 0.05 ); MMP-2 gene−735 (C/T) locus CC genotype and MMP-9 gene−1562 (C/T) locus TT genotype are dangerous genotypes for colorectal cancer. OR values were 1.490 (95% CI: 1.085–2.047), 1.519 (95% CI: 1.061–2.174); TNM stage III-IV, the proportion of CC genotype and TT genotype at MMP-9 gene−1562 (C/T) locus in patients with lymph node metastasis is higher than that without lymph node metastasis of TNM stage I-II patients ( P < 0.05 ); MMP-2 gene in colorectal cancer patients. Serum MMP-2 levels in patients with CC genotype at 735 (C/T) locus were higher than those with CT + TT genotype, and serum MMP-9 levels in patients with TT genotype at MMP-9 gene−1562 (C/T) locus were higher CT + CC genotype patients ( P < 0.05 ). Conclusion. The CC genotype at −735 (C/T) locus of the MMP-2 gene and the TT genotype at−1562 (C/T) locus of the MMP-9 gene are risk genotypes for the development of colorectal cancer.

To explore the genes correlated with the prognosis in colorectal cancer is the main objective of the study. Identification of differential genes through series of GSE20970, GSE37182, GSE44861 and GSE64392 from gene expression Omnibus database and then the differential genes were analyzed by gene ontology and Kyoto encyclopedia of genes and genomes enrichment. Through protein-protein interaction networks, screening of key genes was done and then we analyzed the prognosis of colorectal cancer from the cancer genome Atlas database. We obtained the differential genes through limma screening in series data GSE20970, GSE37182, GSE44861 and GSE64392 from gene expression Omnibus database. Venn diagram was used to screen 206 intersecting differentially expressed genes. Gene ontology enrichment analysis terms used were cytosol, transcription, deoxyribonucleic acid-templated, nucleic acid-templated transcription, ribonucleic acid biosynthetic process, nucleobase-containing compound biosynthetic process, heterocycle biosynthetic process, aromatic compound biosynthetic process, regulation of nucleobase-containing compound metabolic process, regulation of ribonucleic acid metabolic process, organic cyclic compound biosynthetic process. Kyoto encyclopedia of genes and genomes pathway terms were interleukin-17 signaling pathway, bladder cancer, phospholipase D signaling pathway, human cytomegalovirus infection, aldosterone-regulated sodium reabsorption, Kaposi's sarcoma-associated herpesvirus infection, forkhead box O signaling pathway, nuclear factor kappa B signaling pathway, hepatitis B. Protein-protein interaction network has shown 206 key genes. According to the top 50 of betweenness value, we analyzed the prognosis of colorectal cancer from the cancer genome Atlas database. We found Ki-ras2 Kirsten rat sarcoma viral oncogene homolog, C-X-C motif chemokine ligand 8, partner and localizer of breast cancer gene 2, peroxiredoxin 4 and translocase of outer mitochondrial membrane 20 are the key genes which impact prognosis in colorectal cancer. We predict that these genes can promote the survival time of colorectal cancer in future.