Background: Lupin is a herbaceous plant from the legume family whose seed allergens usually have cross-reaction with peanut. Lupin flour is used in human nutrition because of its high nutritional and functional qualities. Aims: The aim of this work was to detect non-specified lupin proteins contained in several manufactured foods. Methods: Serum from a patient suffering anaphylactic episodes after ingestion of a certain brand of cookies and with oral allergy syndrome after eating chicken bouillon was used as a tracer. Lupin seeds and commercial food extracts were analyzed by SDS-PAGE, immunoblotting and immunoblotting inhibition. Lupin extract allergenicity after thermal processing was also analyzed. Results: A lupin allergen with a molecular weight close to 14 kDa was detected in extracts from cookies, a chicken bouillon cube and a chicken dehydrated soup. Conclusions: The presence of unsuspected, hidden non-specified lupin sources in food labeling was demonstrated. According to the results of this study, it is important for food-allergic patients that food labels should declare all the components irrespective of their quantity.

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The prevalence of contact allergy to specific allergens differs among countries, as a result of social and environmental characteristics. Patch testing is the gold standard in the diagnosis of contact dermatitis to identify the culprit substance. TRUE Test ® is a ready-to-use patch test system commercialized since 1985, and is used worldwide. As TRUE Test ® does not cover several allergens recommended for the (European) baseline series, clinicians have to test additional allergens separately. Recently, TRUE Test ® added five new allergens. The newly included allergens are gold sodium thiosulfate, bacitracin, parthenolide, Disperse Blue 106, and 2-bromo-2-nitropropane-1,3-diol (bronopol). The aim of our study was to evaluate the relevance of these new allergens as a part of our baseline series.We included all patients from seven allergy departments in Spain who had been referred for patch testing with the 34-allergen TRUE Test ® , including the five new allergens, and six additional allergens to cover the recommended standard panel, during 1 year. Readings were performed on day (D)2 and D4, according to ICDGR recommendations. Results were analysed as positive versus negative, and the clinical relevance of positive results was evaluated.One thousand and forty-six patients were included. Of these, 66 patients (6.3%) had a positive result with one or more of the five new allergens. Many positive results were obtained with gold sodium thiosulfate (3.2% of the total sample). Readings were +++ in 8 patients, ++ in 11 patients, and + in 14 patients, respectively, and were

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