Potatoes are one of the most responsive crops for grooming techniques since in the period from seedlings before closing rows are easily suppressed by weeds that take away nutrients, water, light from it. The factor that causes high harm to potatoes is weed. By absorbing a large number of nutrients and moisture from the soil, weeds inhibit the growth and development of plants, reducing their potential productivity. The purpose of the study was to establish the effect of the timing of the introduction of bedding manure in conjunction with mineral fertilizers and planting density on the weediness of potato plantings. Studies were provided to identify the impact of the timing of bedding manure together with mineral fertilizers and planting density for weed planting potatoes. Experiments were laid by the systematic method with a tiered arrangement of variants in the experiment with a 3-fold repetition. We used cattle manure on straw bedding for autumn-winter harvesting. The following types of fertilizers were used: ammonium nitrate, double superphosphate, potassium chloride. Determination of the amount and biomass of weeds in potato plantings was carried out in the flowering phase and before harvesting. The application of manure was accompanied by an increase in the number and weight of weeds. Annual weeds predominated in potato plantings: Amaranthus retroflexus L., 1753 and Amaranthus blitoides S. Watson, 1877, Chenopodium album L. Chenopodium album L., 1753, Setaria P. Beauv, 1812 and Setaria viridis (L.) P. Beauv, 1812, Echinochloa crus-galli (L.) BEAUV., 1812 and others, perennials included Cirsium arvense (L.) SCOP. 1772 and Sonchus arvensis L., 1753, (Elytrigia repens (L.) DESV. ex NEVSKI, 1933 and others. Planting density also has a definite effect on the development of weeds. When manure was applied in autumn under the plow, the number of weeds increased in comparison with other options, where only mineral fertilizers were applied. The application of manure over frozen plow and in winter over snow leads to a slight decrease (2.3-2.5 pcs/m2) of weeds. The greatest number of weeds is observed during the spring application of manure for plowing the fall plow. Nevertheless, studies aimed at clarifying the composition and structure of weediness, the number and weight of weeds in potato plantings are of great practical importance for local agricultural producers. These data should contribute to the development of an effective weed control system and high yields of potatoes in the region

The rational use and protection of soils in market conditions requires adequate application of new scientific and methodological approaches. One of such systematic-analytical methods of soil cadastre organizations is a combination of traditional terrestrial methods with technologies of geoinformation systems (GIS) based on extensive use of satellite images in different resolutions. The aggregate of information necessary for mapping soil cover patterns and their quantification has been described in GIS databases. Data integration has been realized through the spatial and attributive component in the form of: the results of topographic and thematic maps. At the same time, the creation of attributive GIS databases involves the digitization of thematic maps tied into a single cartographic projection (as a topographic map with a scale of 1: 50 000). As a result of the study, thematic maps and attributive databases of GIS of soils were formed. As a result of research, based on GIS technology, a digital soil map of the Akshat rural county of the Chingirlau district of the West Kazakhstan region has been developed using the ArcGIS software product.

The genus Ajuga (Lamiaceae) is rich in medicinally important species with biological activities ranging from anti-inflammatory, antitumor, neuroprotective, and antidiabetic to antibacterial, antiviral, cytotoxic, and insecticidal effects. Every species contains a unique and complex mixture of bioactive metabolites—phytoecdysteroids (PEs), iridoid glycosides, withanolides, neo-clerodane terpenoids, flavonoids, phenolics, and other chemicals with high therapeutic potential. Phytoecdysteroids, the main compounds of interest, are natural anabolic and adaptogenic agents that are widely used as components of dietary supplements. Wild plants remain the main source of Ajuga bioactive metabolites, particularly PEs, which leads to frequent overexploitation of their natural resources. Cell culture biotechnologies offer a sustainable approach to the production of vegetative biomass and individual phytochemicals specific for Ajuga genus. Cell cultures developed from eight Ajuga taxa were capable of producing PEs, a variety of phenolics and flavonoids, anthocyanins, volatile compounds, phenyletanoid glycosides, iridoids, and fatty acids, and demonstrated antioxidant, antimicrobial, and anti-inflammatory activities. The most abundant PEs in the cell cultures was 20-hydroxyecdysone, followed by turkesterone and cyasterone. The PE content in the cell cultures was comparable or higher than in wild or greenhouse plants, in vitro-grown shoots, and root cultures. Elicitation with methyl jasmonate (50–125 µM) or mevalonate and induced mutagenesis were the most effective strategies that stimulated cell culture biosynthetic capacity. This review summarizes the current progress in cell culture application for the production of pharmacologically important Ajuga metabolites, discusses various approaches to improve the compound yield, and highlights the potential directions for future interventions.

BACKGROUND: Dicrocoeliasis is caused by trematode Dicrocoelium lanceatum from the family Dicrocoeliidae, a parasite in the bile ducts of the liver of domestic and wild animals. Dicrocoeliasis mainly affects sheep, cattle, camels, zebu, deer, fallow deer, argali, less often – horses, donkeys, dogs, rabbits, hares and bears, as well as humans. Dicrocoeliasis of ruminants is widespread across the whole Kazakhstan. Invasive diseases represent a significant obstacle in the development of domestic maral breeding, among which trematodoses, and particularly dicrocoeliasis of maral play a major role. AIM: The aim of the research was to study the influence of dicrocoelia on the ultrastructural organization of the liver of maral. MATERIALS AND METHODS: For examination under electron microscope, biopsy pieces of liver tissue of maral were fixed in 2.5% solution of glutaraldehyde with post-fixation in 1% solution of osmium tetroxide, conducted according to a conventional method, and enclosed in epon. Semi-thin and ultra-thin sections were prepared on the ultra-microtome Leica. Semi-thin sections were stained with methylene blue, azure 2, and studied at a high-resolution light optical level. The ultra-thin sections were contrasted with uranyl acetate and lead citrate according Reynolds method and examined under electron microscope Libra 120 (C. Zeiss). RESULTS: The light optical examination of half-thin sections revealed that the morphological pattern of pathological changes in liver tissue was polymorphic, even within a single hepatic lobe. CONCLUSIONS: In the liver of maral infected with chronic dicrocoeliasis, dystrophic and destructive pathological changes developed in all the cellular structures of the hepatic lobules: In the form of plethora and vast enlargement of sinusoids, vacuolar and lipodegeneration of hepatocytes, destruction of the hepatic tissue with edema, hemorrhages, in the appearance of cells associated with inflammation, and the deposition of hematin crystals.