Screening methods to identify methicillin-resistant Staphylococcus aureus (MRSA) were compared using 96 isolates representing 17 distinct clones. The sensitivity of four commercial agglutination tests was determined in comparison to the tube coagulation test, and the results related to the presence of the coagulase gene. The broth screening test, agar dilution test and disc diffusion test were carried out, and the results related to the presence of the mecA gene. Mannitol salt agar and Iso-Sensitest agar with varying salt supplements were used. All agglutination tests had high rates of detection of Staphylococcus aureus (95.8-99.0%). Resistance in mecA gene-positive Staphylococcus aureus isolates was correctly detected by the oxacillin broth test, the agar dilution test and the disc diffusion test on mannitol salt agar, whereas on Iso-Sensitest agar detection rates were lower (between 68.5% and 94.4%, depending on the salt supplement). Incubation of the Iso-Sensitest plates for 48 hours significantly improved the rate of detection of resistance, but increased the major error rate up to 71.4%. MecA gene-positive Staphylococcus aureus isolates not detected by the disc diffusion test on Iso-Sensitest agar had significantly lower oxacillin minimal inhibitory concentration values and were significantly less resistant to a variety of antibiotics. Thus, mannitol salt agar might be a suitable medium for use in the disc diffusion and agar dilution test to detect resistance to oxacillin in Staphylococcus aureus.
We prove that every Legendrian knot in the tight contact structure of the 3-sphere is determined by the contactomorphism type of its exterior. Moreover, by giving counterexamples we show this to be not true for Legendrian links in the tight 3-sphere. On the way a new user-friendly formula for computing the Thurston-Bennequin invariant of a Legendrian knot in a surgery diagram is given.
It is known that any contact 3-manifold can be obtained by rationally contact Dehn surgery along a Legendrian link L in the standard tight contact 3-sphere. We define and study various versions of contact surgery numbers, the minimal number of components of a surgery link L describing a given contact 3-manifold under consideration.In the first part of the paper, we relate contact surgery numbers to other invariants in terms of various inequalities. In particular, we show that the contact surgery number of a contact manifold is bounded from above by the topological surgery number of the underlying topological manifold plus three.In the second part, we compute contact surgery numbers of all contact structures on the 3-sphere. Moreover, we completely classify the contact structures with contact surgery number one on S 1 × S 2 , the Poincaré homology sphere and the Brieskorn sphere Σ(2, 3, 7). We conclude that there exist infinitely many non-isotopic contact structures on each of the above manifolds which cannot be obtained by a single rational contact surgery from the standard tight contact 3-sphere. We further obtain results for the 3-torus and lens spaces.As one ingredient of the proofs of the above results we generalize computations of the homotopical invariants of contact structures to contact surgeries with more general surgery coefficients which might be of independent interest.
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