In this article, we summarize the work of the NASA Outer Planets Assessment Group (OPAG) Roadmaps to Ocean Worlds (ROW) group. The aim of this group is to assemble the scientific framework that will guide the exploration of ocean worlds, and to identify and prioritize science objectives for ocean worlds over the next several decades. The overarching goal of an Ocean Worlds exploration program as defined by ROW is to “identify ocean worlds, characterize their oceans, evaluate their habitability, search for life, and ultimately understand any life we find.” The ROW team supports the creation of an exploration program that studies the full spectrum of ocean worlds, that is, not just the exploration of known ocean worlds such as Europa but candidate ocean worlds such as Triton as well. The ROW team finds that the confirmed ocean worlds Enceladus, Titan, and Europa are the highest priority bodies to target in the near term to address ROW goals. Triton is the highest priority candidate ocean world to target in the near term. A major finding of this study is that, to map out a coherent Ocean Worlds Program, significant input is required from studies here on Earth; rigorous Research and Analysis studies are called for to enable some future ocean worlds missions to be thoughtfully planned and undertaken. A second finding is that progress needs to be made in the area of collaborations between Earth ocean scientists and extraterrestrial ocean scientists.

We describe the history and features of the Ladder of Life Detection, a tool intended to guide the design of investigations to detect microbial life within the practical constraints of robotic space missions. To build the Ladder, we have drawn from lessons learned from previous attempts at detecting life and derived criteria for a measurement (or suite of measurements) to constitute convincing evidence for indigenous life. We summarize features of life as we know it, how specific they are to life, and how they can be measured, and sort these features in a general sense based on their likelihood of indicating life. Because indigenous life is the hypothesis of last resort in interpreting life-detection measurements, we propose a small but expandable set of decision rules determining whether the abiotic hypothesis is disproved. In light of these rules, we evaluate past and upcoming attempts at life detection. The Ladder of Life Detection is not intended to endorse specific biosignatures or instruments for life-detection measurements, and is by no means a definitive, final product. It is intended as a starting point to stimulate discussion, debate, and further research on the characteristics of life, what constitutes a biosignature, and the means to measure them.

This year marks the 50(th) anniversary of a proposal by Alex Rich that RNA, as a single biopolymer acting in two capacities, might have supported both genetics and catalysis at the origin of life. We review here both published and previously unreported experimental data that provide new perspectives on this old proposal. The new data include evidence that, in the presence of borate, small amounts of carbohydrates can fix large amounts of formaldehyde that are expected in an environment rich in carbon dioxide. Further, we consider other species, including arsenate, arsenite, phosphite, and germanate, that might replace phosphate as linkers in genetic biopolymers. While linkages involving these oxyanions are judged to be too unstable to support genetics on Earth, we consider the possibility that they might do so in colder semi-aqueous environments more exotic than those found on Earth, where cosolvents such as ammonia might prevent freezing at temperatures well below 273 K. These include the ammonia-water environments that are possibly present at low temperatures beneath the surface of Titan, Saturn's largest moon.

Inspired by the recent results of the Dawn mission, thermodynamic models of rock alteration and brine evaporation have been used to help understand the conditions under which water-rock interaction took place within the dwarf planet Ceres. This analysis constrains Ceres's early history and offers a framework within which future observations may be interpreted. A broad range of alteration conditions have been simulated using the Geochemist's Workbench and PHREEQC software, associated with the FREZCHEM model that constrains the consequences of freezing the liquid phase in equilibrium with the observed mineralogical assemblage. Comparison of the modeling results with observed surface mineralogy at Ceres indicates advanced alteration under a relatively high fugacity of hydrogen, a conclusion that is consistent with predictions for, and observations of, large icerich bodies. The simulations suggest production of methane that could help regulate the redox environment and possibly form clathrate hydrates upon freezing of the early ocean. The detection of localized occurrences of natrite (sodium carbonate) at the surface of Ceres provides key constraints on the composition of fluids that are necessarily alkaline. In addition, the combined hydrothermal and freezing simulations suggest that hydrohalite may be abundant in Ceres's subsurface, similar to Earth's polar regions. The global homogeneity of Ceres's surface, made of material formed at depth, suggests a large-scale formation mechanism, while local heterogeneities associated with impact craters and landslides suggest that some form of sodium carbonate and other salts are accessible in the shallow subsurface.