[3H]R5020 was bound to cytosolic and nuclear samples of human Fallopian tube with high affinity and specificity. The cytoplasmic and nuclear concentrations of progestagen receptor varied, throughout the menstrual cycle, in the ampulla, isthmus and fimbria. Concentrations were higher at the late proliferative stage of the cycle than at the early proliferative and late secretory stages. A positive linear regression was observed between cytosolic and nuclear progestagen receptor concentrations and plasma oestradiol levels. A negative linear relationship was observed between cytosolic progestagen receptor concentration and plasma progesterone levels during the secretory stages of the menstrual cycle.

Summary31 patients presenting a Major Depressive Episode were divided into two groups (endogenous versus neurotic depression), in keeping with the Newcastle criteria (Garside and Roth, 1974). 10 patients were allocated to each group for the realization of a test of growth hormone (GH) response to clonidine stimulation.The patients received no psychotropes for 8 days prior to the test. Mean age for the neurotic and endogenous groups was respectively 36.67 ± 3.18 years and 44.71 ± 2.56 years. Severity of depression, assessed with the Hamilton rating scale (21 items), was comparable in the two groups (35.35 ± 4.12 versus 39.8 ± 6.13).The test was carried out in two phases in each patient. During the initial phase, saline was infused over 10 minutes and continuous sampling was realized over a 4-hour period (automatic fraction collector with peristaltic pump), at between 9 AM and 1 PM. 48 hours later, the same procedure was repeated with the addition of 15 µg clonidine. This procedure allowed partial neutralization, for interpretation of results, of the «test apprehension» effect. Assays were carried out by radioimmunoassay (pooled samples), and correspond to real values for 10-minute time intervals (integrated values).Spontaneous GH secretion in the endogenous group was significantly lower (0.57 ± 0.16 ng/ml) than in the neurotic group (5.03 ± 1.08 ng/ml) and the control group (2.47 ± 0.78 ng/ml). After clonidine stimulation, GH response in the neurotic group was identical to that in the control group. No significant response was observed in the endogenous group. These results confirm those of several previous studies (Matussek, Charney, Checkley, Boyer, Corn, Siever) and seem to indicate hyposensitivity of the post-synaptic α2-adrenergic receptors in endogenous depression. Nevertheless, spontaneous GH hyposecretion in the same patients necessarily involves other mechanisms. The hypotheses concerning these mechanisms will be discussed in the second part of this study.

Background: Recruitment of older adults into aging research is a challenge. Several research studies and clinical trials have failed due to low participant enrollment. Older adults face many barriers to study participation (mistrust of research, lack of transportation, health status, etc.), so special efforts must be made to recruit them into research. We created a recruitment questionnaire with the goal of understanding how to better promote the study and to obtain participant suggestions of groups and locations where the study team could recruit new participants. Method:The Health and Aging Brain Study: Health Disparities (HABS-HD) is a community based research study of cognitive aging in diverse communities. We designed a voluntary exit questionnaire for current study participants regarding their recruitment preferences. The questionnaire included the following questions: 1) How did you find out about the study? 2) How can we promote the study to someone similar to you?3)Which of the following advertising methods do you prefer? 4) Do you know any organization/group that would benefit from the study information? Questionnaire data was compiled and analyzed to assess recruitment preferences and suggestions. Result:The total number of participants who responded to the questionnaire was 413. 26.9% of respondents stated that they would prefer TV as their main advertising method, followed by the internet (23%) and postal service (mail) (22.7%). Word of mouth was listed as the best way to promote the study (26.9%) followed by community engagement (16.2%) and church (8.2%). When asked for names of organizations, participants recommended tradition groups such as churches, senior centers, and retirement homes. Participants also recommended groups such as rotary clubs, veterans associations, libraries, and the community college senior education program. Conclusion:Participants can assist in recruitment efforts through suggesting organizations that study personnel may not have reached out to. The input allows for assessment of recruitment and the suggestions can broaden the study's reach.

Background Medical comorbidities are commonly associated with poorer cognition. Hypertension and depression are common comorbidities linked to cognitive decline and Alzheimer’s disease (AD). Mexican Americans experience higher rates of these medical comorbidities and also present with cognitive decline at younger ages as compared to their non‐Hispanic White counterparts. Increased work has expanded to look at reasons for increased risk for neurodegenerative conditions such as AD specifically among this population. The purpose of this study was to examine the role of co‐morbid hypertension and depression on cognitive and proteomic outcomes. Method Data from 548 Mexican Americans (415 normal control, 133 MCI/AD) was collected from a community based epidemiological study of aging. The average age was 61 and the average education was 7.7 years. Participants were stratified into 4 groups: no hypertension or depression (N= 101), hypertension only (N= 218), depression only (N=59), and comorbid hypertension and depression (N=170). One‐way ANOVAs were utilized to examine group differences in cognitive performance and proteomics. Cognition was assessed via WMSIII Logical memory 1 & 2, Trails A/B, EXIT, and MMSE. Biomarkers of inflammation (serum TNFα, IL5, IL6, IL7, IL10 and CRP) and neurodegeneration (plasma Aß42/ Aß40, Tau, NFL) were analyzed. A logistic regression was calculated with normal control versus MCI/AD as the outcome variable and comorbid condition, education and age as predicting variables. Result In normal controls, comorbid hypertension and depression was significantly associated with poorer executive and global cognitive function; and this comorbidity increased risk of MCI/AD diagnosis (OR= 1.950; 95/5 CI= 1.23‐3.08). Among normal controls, the comorbid group was found to have significantly higher levels of NFL (p<0.032) and IL6 (p<0.018) than the depression only group. Among those with MCI/AD, the comorbid group had significantly higher NFL levels (p<0.002) than the neither condition group. Conclusion The results of the study demonstrated that comorbid hypertension and depression was related to poorer cognitive functioning and increased risk for cognitive impairment among Mexican Americans. Findings support that medical conditions likely influence biomarkers of inflammation and neurodegeneration, which lends support for a precision medicine approach to treating MCI and AD specifically among minority populations.