<p><strong> Objective</strong>. To determine amylolytic, cellulolytic, lipolytic, pectinolytic and proteolytic activities in 32 <em>Fusarium</em> spp. isolates from humans, animals and plants. <strong>Materials and methods</strong>. Qualitative determination of enzymatic activities was done by measuring hydrolysis halos in agar plates with their corresponding substrate. Quantitative determination was done by colorimetric techniques, using liquid culture supernatants to determine the respective substrate degradation. <strong>Results</strong>. All isolates showed enzymatic activities from a qualitative point of view, except amylolytic and lipolytic. Quantitative determination was possible for all the evaluated enzymes except lipases. <strong>Conclusion.</strong> The determination of amylolytic, cellulolytic, pectinolytic and proteolytic enzymatic profiles of each of the <em>Fusarium</em> isolates assessed suggests their capacity to degrade these substrates, irrespectively of their origin.</p> <p><strong>Key words</strong>: <em>Fusarium</em>, multihost pathogen, amylases, celluloses, pectinases, proteases, lipases</p> <p><strong> </strong></p><br />