The colony structure of Oecophylla smaragdina within the compound of Universiti Sains Malaysia, Penang Island, Malaysia was determined. The current study involved a total of twelve nests which were taken from two different locations. Each nest was dissected and the specimens were separated according to their caste. Results show that the colony structure of O. smaragdina consisted of a dealate queen, eggs, larvae of female alates, pupae of major and minor workers, pupae of female alates, major workers, minor workers, female alates and male alates. Our present study shows that O. smaragdina had a distinct caste system and the numbers of individuals in each caste reflect their respective functions which contributed to the success of their colony.

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Highlights• In this study, the weaver ants were nocturnal and foraged more intensively between 2 a.m. and 6 a.m.• In the case of food preference, the weaver ants highly preferred tuna followed by chicken skin and milk.• The foraging activity of weaver ants was influenced by both temperature and relative humidity. Abstrak: Kerengga (Oecophylla smaragdina) adalah satu komuniti serangga yang penting di kanopi pokok. Walau bagaimanapun, kajian tentang aktiviti pencarian makanan kerengga adalah terhad. Oleh sebab itu, satu kajian lapangan dijalankan untuk mengkaji pemilihan makanan dan aktiviti pencarian makanan oleh semut ini dijalankan selama tiga hari. Pemilihan makanan oleh kerengga ini dikaji dengan menggunakan tiga jenis makanan (tuna, kulit ayam, dan susu tepung) yang mengandungi protein, lipid, dan karbohidrat. Aktiviti pencarian makanan dikaji berdasarkan dua parameter iaitu suhu persekitaran dan kelembapan relatif yang direkod sepanjang tempoh experimen. Keputusan experimen menunjukkan O. smaragdina lebih menggemari makanan berprotein berbanding makanan berlipid dan berkabohidrat, serta aktiviti pencarian makanan dipengaruhi oleh suhu persekitaran dan kelembapan relatif. Hasil kajian ini menunjukkan bagaimana kerengga bertindak balas terhadap tiga jenis makanan yang berbeza dan secara tidak langsung, menunjukkan aktiviti pencarian makanan yang strategik untuk memaksimumkan bekalan makanan kepada koloni mereka. Kata kunci: Oecophylla smaragdina, kerengga, koloni, pilihan makanan, aktiviti pencarian makanan Abstract: Weaver ant (Oecophylla smaragdina ) is an important insect community in tree canopies, but little is known about their foraging behaviours. Therefore, a field experiment was conducted in order to assess the food preferences and foraging activity of weaver ants for three consecutive days. The food preferences of the weaver ants were evaluated using three types of foods (tuna, chicken skin, and milk powder) containing varying contents of protein, lipid, and carbohydrate. The foraging activity was examined based on two parameters, namely temperature and relative humidity which were recorded throughout the study. Results revealed that food higher in protein content was highly preferred by the * Corresponding author: marcela.fun@gmail.comMarcela Pimid et al. 168O. smaragdina compared to foods with lipid and carbohydrate contents, and the foraging activity of the O. smaragdina was significantly influenced by both temperature and relative humidity. The present study exhibits how the weaver ants respond to different types of foods and indirectly, forming a strategic foraging activity to maximise their food supplies for their colony.

Effective conservation measures are essential to protect nature and wildlife which act as flagship attraction for ecotourism to flourish. The study focuses on conservation management of natural forests and wildlife by multiple stakeholders in Lower Kinabatangan Sabah. Rare and endangered wildlife species is the main attraction for ecotourism development in this area. The study employed a mixed method research by integrating quantitative and qualitative approaches. Analyses revealed contradictory findings whereby local indigenous communities were unaware about progress of established Lower Kinabatangan Wildlife Sanctuary and thereby requested for enhancing current conservation management. However, other stakeholders reported that the sanctuary fulfilled its objectives. The study further proposed solutions to improve current conservation by considering the viewpoints of all stakeholders involved in conservation initiatives in the Lower Kinabatangan Sabah.

The mosquito Aedes aegypti is the primary vector of the dengue, yellow fever, and chikungunya viruses. Evidence shows that Ae. aegypti males are polyandrous whereas Ae. aegypti females are monandrous in mating. However, the degree to which Ae. aegypti males and females can mate with different partners has not been rigorously tested. Therefore, this study examined the rates of polyandry via parentage assignment in three sets of competitive mating experiments using wild-type male and female Ae. aegypti. Parentage assignment was monitored using nine microsatellite DNA markers. All Ae. aegypti offspring were successfully assigned to parents with 80% or 95% confidence using CERVUS software. The results showed that both male and female Ae. aegypti mated with up to 3–4 different partners. Adults contributed differentially to the emergent offspring, with reproductive outputs ranging from 1 to 25 viable progeny. This study demonstrates a new perspective on the capabilities of male and female Ae. aegypti in mating. These findings are significant because successful deployment of reproductive control methods using genetic modification or sterile Ae. aegypti must consider the following criteria regarding their mating fitness: 1) choosing Ae. aegypti males that can mate with many different females; 2) testing how transformed Ae. aegypti male perform with polyandrous females; and 3) prioritizing the selection of polyandrous males and/or females Ae. aegypti that have the most offspring.