Chronic wounds are difficult to heal due to several forms of infections. This issue can be addressed through the utilization of advanced approaches such as regenerative medicine and tissue engineering. One possible strategy could be the modification of bacterial cellulose (BC) structure to increase the current efforts of building scaffolds for tissue engineering. The current study was aimed to make structural comparative analysis of paraffin-altered BC and synthesis of its composite with alginate. Porous BC was produced through the addition of paraffin particles. Thereafter, a three dimensional scaffold of porous BC with alginate (BC/AL) was fabricated through the blending of BC and AL solutions. The paraffin particles were incorporated in the process of culturing Acetobacter xylinum for BC pellicle formation. Structural features of porous BC and BC/AL scaffold paralleled with original BC were investigated by the scanning electron microscope (SEM). SEM analysis revealed that porous BC possessed large surface (micro-scale pores) while the BC/AL scaffolds demonstrated a well-connected porous network. The purity and chemical structure of surfactants (Span 80 and OP-10) treated BC/AL scaffold was determined by Fourier transform infrared spectrum (FTIR) which confirmed the presence of any impurity in the form of paraffin in the 3D network of scaffold. Average water absorption analysis showed that pristine BC, porous BC, and BC/AL scaffold possessed 99.62%, 97.61%, and 96.94% water, respectively. Mechanical characteristics analysis of pristine BC, porous BC, Al, and BC/AL showed that these were conserved at certain levels between BC and AL. The MTT assay using RPMI 1640 medium (Hyclone, USA) supplemented with 10% fetal bovine serum (Gibco, USA) were used for human fetal hepatocyte L-02 cell line (Tongji Hospital, Wuhan, China) culture. Cells were incubated at 37 o C, 5% CO2 and humid atmosphere. The scaffold developed in the current study can serve as possible material for the treatment of burns and chronic wounds treatment.

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Background: The majority of pregnancies and births proceed without complications. However, all pregnancies present risks of complications that could endanger the maternal prognosis. One of the most serious of these complications is the bleeding during delivery. It’s often unpredictable nature, when it occurs, involves an urgent obstetric situation which requires early diagnosis and rapid and effective follow-up. The general objective of this study was to assess the impact of the introduction of the active follow-up care of the third phase of childbirth in the prevention of bleeding during delivery with our place of study, the obstetrical gynecology department of Kindia regional hospital (Konacri Guinea), with the aim of optimizing the follow-up care of pregnant women during their childbirth. Methodology: We carried out a case-control study with both retrospective and prospective data collection on the records of participants with cases of hemorrhage during delivery in the gynecology department of the Kindia regional hospital. Retrospective data collection covered a period of 25 months before the active follow-up care of the third stage of childbirth and concerned the control group of study participants, while the one of prospective data covered a period of 19 months after the introduction of the active follow-up care of the third stage of childbirth from the 1st January 2019 to 30th September 2020 and concerned the cases group. The criteria for matching these two groups of study participants were the diagnosis and the follow-up of the delivery hemorrhage. Results: During our study period in the gynecology department of the Kindia regional hospital, we identified 223 cases of delivery hemorrhage out of 2198 deliveries before the period of introduction of the active follow-up care of the third stage. This represents an incidence of 10.14%, compared to 73 cases of delivery hemorrhage out of 2714 deliveries during the period of the active follow-up care of the third stage. For an incidence of 2.68%. We found that the hemorrhages during delivery did not spare any age group and were observed for all parities. There were common reasons for admission before and after the integration of the active follow-up care of the third stage of childbirth namely vulvar hemorrhage and physical asthenia, which were reflected in the clinical picture of all cases bleeding from the issue. In addition, headaches, dizziness, and thirst were found, but in different proportions and significantly less frequent since the introduction of the active follow-up care of the third stage of childbirth. This sign were in this context the consequence of the state of shock with respective frequencies of 46.63% and 28.76% before and after the introduction of the active follow-up care of the third stage of childbirth and anemia was present in all cases of delivery hemorrhage. Conclusion: The introduction of active follow-up care of the third stage of childbirth has reduced the frequency of delivery hemorrhages and may therefore help to reduce maternal morbidity and mortality.

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