Crude extract of cultures of 13 fungal strains identified as Phomopsis sp. and isolated as endophytes from the leaves of Aspidosperma tomentosum and twigs of Spondias mombin were examined for their antibacterial and antifungal activities. The screening was conducted using the bioautographic TLC agar-overlay technique against bacteria (E. coli, P. aeruginosa, S. aureus), yeast (C. albicans, S. cerevisiae), and readily adapted for use with filamentous fungi (A. niger, F. oxysporum). Three of the 13 extracts effectively inhibited the growth of all test-organisms, indicating that they may represent a potential for pharmaceutical and/or agricultural applications and are worthy of further study.