We report the estimated allele frequencies for 13 and 14 microsatellite loci in two populations of Minas Gerais, Brazil as follows: Belo Horizonte (the capital) and Marinhos (an African-derived community). Analysis of the African, Amerindian, and European genetic contributions to both populations, together with historical information, revealed distinct differences between the two populations. Estimates for Belo Horizonte revealed a higher-European (66%) than African (32%) contribution, and a minimal Amerindian contribution. These results are consistent with the peopling of the city mainly by people from the Minas Gerais hinterland, a people highly admixed but with more European ancestry. Estimates for Marinhos confirmed the high-African component of the population. However, a temporal analysis of two datasets-CURRENT (representing the population living in Marinhos today) and ORIGINAL (representing families, who have lived in Marinhos since the onset of the 20th century),-identified a diminishing of the population's African ancestry from 92% in the ORIGINAL group to 67% in the CURRENT group. This change is here interpreted as a consequence of the growing migration into the village of people with more European ancestry and subsequent admixture with the local population.
. The patients were preferentially treated with ovulation induction with gonadotropins. With the addition ofgonadotropin stimulation, the total and term pregnancy rates per cycle were 14% and 1 1%, respectively, includingalletiologicfactors. These rates were improvedoverthe3%and 2.6%ratesreported inour previousstudy inwhichovarianstirnulationwasnotgenerallyused. Inniale factor patients, the term pregnancy rate was 9%, higher than the 4% term pregnancy rate reported in our previous study. In the present series, morphology was the only severely impaired parameter. The term pregnancy rate was 1 1 % for patients with ovulatory dysfunction, 10% for those with cervical factor, and 10.5% for those with unexplained infertility.
Due to their ease of analysis and high informativity, these new STR multiplexes will be useful for extending current marker sets for forensic and paternity purposes.
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