The importance of copper as an essential element can be estimated by the wide range of copper proteins and enzymes playing different roles in biological systems. In the last decades many bioinorganic studies were developed on mimetic complexes of copper-dependent proteins, in order to verify the interrelations between structural and functional properties of active copper centers. Among the most studied copper ion ligand, diimine compounds have deserved special attention due their flexibility, facility of preparation, and ability to stabilize both oxidation states of this metal. In our laboratory, we have been investigating some Schiff base copper complexes as mimics of different proteins, with emphasis on functional aspects, trying to elucidate mechanisms of reaction, based on proposed intermediary species, in addition to molecular shapes. Particularly, mimics of the copper-zinc superoxide dismutase, and of monooxigenases and oxidases exhibiting dicopper sites are discussed in this work.

99mTc is the most applied medical radioisotope in the world, especially for cancer diagnosis procedures. It is provided by 99Mo radioactive decay, which is one of the fission products from the uranium irradiation in nuclear reactors. At the main production plants, the 99Mo chemical processing may be lined up in several steps to separate it from other fission products according to the features of the used targets or the local requirements as well. In this work, two routes of 99Mo purification, MR1, and MR2, were purposed as an alternative to be set up in the Brazilian Multipurpose Reactor project (BMR). The MR1 route was performed by three chromatographic columns packed with Dowex 1x8 resin, Chelex resin, and alumina, respectively. The route MR2 was carried out also by chromatography applying two columns filled with Dowex 1x8 and alumina respectively, but including a sublimation process performed in a tubular oven under programmed conditions. The final yield for the MR1 route was 84.4 % and the overall time process was about 7 hours, whereas the MR2 route reached 75.3 % in 9 hours.

pela amizade, companheirismo e brincadeiras do diaa-dia. Aos companheiros de laboratório: Christian, Melina, Adriana, André, Maria Aparecida, Eduardo e Nara pela amizade e convívio. Ao professor Henrique Toma e a Dra. lzilda Bagatin pela realização de alguns experimentos. Aos colegas, professores e funcionários do Instituto de Química da USP que de alguma forma colaboraram na realização deste trabalho. Ao Professor Jivaldo Mattos e a Maria Luiza pela possibilidade de realizar os experimentos de IV e termoanálise. Ao José Soares pela realização das análises de cobre. Aos amigos Hector, Fátima e Maria Cristina pelo incentivo e amizade. À FAPESP pela bolsa concedida.

DAMASCENO, Marcos O. Study of purification routes in production processes of fission Mo-99 and I-131. 2019. 160 p. Tese (Doutorado em