This study aimed to test an alternative protocol with human plasma to control Trypanosoma evansi infection in mice. Plasma from an apparently 27-year-old healthy male, blood type A+, was used in the study. A concentration of 100 mg.dL-1 apolipoprotein L1 (APOL1) was detected in the plasma. Forty mice were divided into four groups with 10 animals each. Group A comprised uninfected animals. Mice from groups B, C and D were inoculated with a T. evansi isolate. Group B was used as a positive control. At three days post-infection (DPI), the mice were administered intraperitoneally with human plasma. A single dose of 0.2 mL plasma was given to those in group C. The mice from group D were administered five doses of 0.2 mL plasma with a 24 hours interval between the doses. Group B showed high increasing parasitemia that led to their death within 5 DPI. Both treatments eliminated parasites from the blood and increased the longevity of animals. An efficacy of 50 (group C) and 80% (group D) of human plasma trypanocidal activity was found using PCR. This therapeutic success was likely achieved in the group D due to their higher levels of APOL1 compared with group C.
Relata-se, dois casos, sendo que no primeiro, realizou-se cirurgia de OVH eletiva. Como MPA,utilizou-se 2,5 mcg/kg de cloridrato de dexmedetomidina e 0,2 mg/kg de cloridrato de metadonaIM. A recuperação anestésica foi tranquila e sem complicações e o animal foi extubado apóscinco minutos, recebendo alta do centro cirúrgico 30 minutos depois. No segundo caso,realizou-se cirurgia de sepultamento da glândula da terceira pálpebra. Como sedativo, utilizouse 3,5 mcg/kg de cloridrato de dexmedetomidina e, como analgésico, 0,2 mg/kg de cloridratode metadona IM. Ao término do procedimento cirúrgico, administrou-se 0,4 mcg/kg decloridrato de atipamezole IM. Decorridos 15 minutos o paciente recuperou-se completamente,podendo ser encaminhado para alta cirúrgica. Os dois procedimentos transcorreram comvalores de PAS e bradicardia elevados,efeitos característicos do uso da dexmedetomidina. Noprimeiro caso a PAS se manteve entre 150 e 180 mmHg, no segundo, entre 160 e 180 mmHg.A partir dos casos relatados, conclui-se que o emprego da dexmedetomidina foi benéfico,proporcionando adequada medicação pré-anestésica e sedação nos referidos pacientes.
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