Proposed environmental flow regime changes downstream of a major water supply dam have been assessed in terms of effects on depth, velocity and fish passage across natural, gravel-bed riffles and rapids. This study focussed on passage requirements for Australian bass, Macquaria novemaculeata (Perciformes, Percichthyidae), a catadromous fish of considerable ecological and recreational fishing importance. Some 23 major riffles and rapids occur between the dam and the tidal limit over a river length of 25 km. Reconnaissance investigations of riffle slope, length, width, depth and morphologic characteristics indicated that wideshallow, steep-turbulent and bifurcating riffle morphologies were most likely to cause problems for upstream bass passage under low flow conditions. Two approaches were used to investigate riffle depths and velocities over a range of flows. A rapid assessment approach directly measured thalweg depths and velocities under two controlled flow release rates in riffles identified as being potentially problematic to upstream bass passage. Detailed topographic surveys and two dimensional hydraulic modelling with River2D was undertaken for two riffles identified as 'worst case' examples of wide-shallow and steep-turbulent morphologies. Results from both approaches were consistent and complementary. Both approaches identified riffles where minimum depths and maximum velocities were likely to be problematic for upstream passage by Australian bass at a flow rate of 130 MLd À1 (the current regulated flow release) but were mitigated at flow rates above 300 MLd À1. Assessment of environmental flow regime transparency and translucency threshold options with regard to a 300 MLd À1 target flow indicated that options where the transparency threshold was set at the 80th flow duration percentile (flows equalled or exceeded for 80% of time), and varied according to the monthly pattern of natural flows, improved hydraulic conditions for upstream bass migration.

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Procedures for impact assessment, including "beyond-BACI" (before–after control–impact) and proportional differences (ratios between impact and control treatments) were used to test population replenishment of marine invertebrates at a marine conservation area (MCA) and three fished (control) areas in the Solomon Islands of the southwestern tropical Pacific. Within shallow reef terrace habitat, the MCA caused abundance and size of the topshell Trochus niloticus to increase but did not affect holothurians (sea cucumbers) or the giant clam Tridacna maxima. Abundance of the nonexploited topshell Tectus pyramis was unchanged at the MCA but increased at the controls, possibly because of changes in abundance of T. niloticus. Within deep slope habitat, the MCA caused increased abundance of the sea cucumber Holothuria fuscogilva and prevented possible declines in abundances of Thelanota anax and all holothurians combined but had no effect on abundances of Holothuria atra or Holothuria fuscopunctata. Power analysis comparing the MCA with controls indicated that further, relatively modest increases in abundance or size of some species would have a good chance of being detected statistically. The beyond-BACI procedure holds promise for enabling rigorous evaluation of marine reserves as management tools at different spatial scales; the use of proportional differences is simpler but has limited management value.

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