Levofloxacin is a member of the fluoroquinolone class of antibacterial agents with a safe and effective treatment. The aim of this study is to evaluate the susceptibility and resistance pattern of clinical isolates causing different types of infections to Levofloxacin. In vitro antibacterial activity and resistance patterns of levofloxacin antibiotic were studied using disk diffusion method. A total 485 strains isolated from various clinical specimens from September 2020 to November 2020 in Al Saleem Medical Laboratory. This is for isolation and identification of pathogenic gram negative and gram positive bacteria. The samples were involving urine, semen, swabs, High vaginal swab, Blood, Stool, CSF, Body Fluid and Sputum. The isolates were identified by biochemical tests as well as the sensitivity and resistant were tested by levofloxacin antibiotic. In present study, gram negative bacteria were prevalent, 75.6% than gram positive bacteria 47.7%. In the four hundred eighty five clinical isolates comprising of Escherichia coli, Klebseilla spp, Staph Aureus, Enterobacter spp, Streptococcus pneumonia, Pseudmonas spp, Streptococcus pyogen, Citrobacter spp, proteus spp and Staph epidermidis were collected from different clinical specimens. The predominantly isolated bacteria were Escherichia coli, followed by Klebseilla spp, staph Aureus and Enterobacter spp. The levofloxacin antibiotic has sensitive against most of the isolates, E Coli (31%), Klebseilla spp (14%), Staph Aureus (8.7), Enterobacter spp (6%), strep pneumonia (5%), Pseudomonas spp (3%), Strep pyogen (1%) and Citrobacter spp (0.6%). Levofloxacin exhibited statistically significantly high bactericidal activity against all strains of gram positive and gram negative bacteria. Levofloxacin was shown to be active against both Gram-positive and Gram-negative bacteria.

Wound infections and septicemia with drug-resistant bacteria lead to higher mortality and morbidity and increased healthcare costs. We aimed to evaluate the prevalence rate of nosocomial bacterial agents with antimicrobial susceptibility patterns in hospitalized and non-hospitalized patients. Samples are collected from the wound pus, burn; medical device tip and blood of male and female patients and are sent for culture and sensitivity to the Microbiology Department of Al Saleem medical laboratory, Benghazi, Libya, from October 2021 to January 2022. Collected blood samples were directly inoculated into brain heart infusion (BHI) broth, while various swabs were collected by Transport medium and both are cultured on Blood agar, Chocolate agar, MacConkey - aerobic and anaerobic blood agar at 35°C, with CO2. The wound pus was the most samples from which bacteria were isolated, followed by medical device tip swabs, and the least isolation was from a blood specimen. 47 of the specimens were culture-positive. Of these Staph aureus, Strep pneumonia and E. coli were the most abundant bacterial identified in clinical samples. According to susceptibility testing results, antibiotic resistance patterns of Gram-negative bacteria showed that the highest resistance rate was against Septrin (74.4%) and the lowest rate, 17.8%, was related to Doxycycline (44.6%) respectively. The isolates from the nosocomial infection in these patients are resistant to Septrin and Doxycycline antibiotics. We recommend that whenever the diagnosis of nosocomial infection is made, a more effective antibiotic treatment be instituted until the susceptibility of the strain is identified.