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Microalgae are unicellular photosynthetic organisms that can be grown in artificial systems to capture CO2, release oxygen, use nitrogen- and phosphorus-rich wastes, and produce biomass and bioproducts of interest including edible biomass for space exploration. In the present study, we report a metabolic engineering strategy for the green alga Chlamydomonas reinhardtii to produce high-value proteins for nutritional purposes. Chlamydomonas reinhardtii is a species approved by the U.S. Food and Drug Administration (FDA) for human consumption, and its consumption has been reported to improve gastrointestinal health in both murine models and humans. By utilizing the biotechnological tools available for this green alga, we introduced a synthetic gene encoding a chimeric protein, zeolin, obtained by merging the γ-zein and phaseolin proteins, in the algal genome. Zein and phaseolin are major seed storage proteins of maize (Zea mays) and bean (Phaseolus vulgaris) that accumulate in the endoplasmic reticulum (ER) and storage vacuoles, respectively. Seed storage proteins have unbalanced amino acid content, and for this reason, need to be complemented with each other in the diet. The chimeric recombinant zeolin protein represents an amino acid storage strategy with a balanced amino acid profile. Zeolin protein was thus efficiently expressed in Chlamydomonas reinhardtii; thus, we obtained strains that accumulate this recombinant protein in the endoplasmic reticulum, reaching a concentration up to 5.5 fg cell-1, or secrete it in the growth medium, with a titer value up to 82 µg/L, enabling the production of microalga-based super-food.

Civil War', pp. 145-168), analyses the political violence and the violent practices operated by the collaborating Fascist government. She observes that this kind of actioneven involving civilians was aimed at striking the 'enemy within': partisans and everyone connected directly or indirectly to the various Resistance movements. The core of Luca Baldissara's chapter ('Il massacro come strategia di guerra, la violenza come forma di dominio dello spazio' ('Massacre as a War Strategy, Violence as a Form of Space Dominance', pp. 169-195) is a deep reflection on 'the instrumental rationality, at a military level, of the extensive use of massacre by German troops in Italy' (p. 168). According to the author, 'the need for political and military control of territory, the urgency to activate effective counterguerrilla tactics, a specific military culture, converge in the period 1943-1945 to define a warlike behaviour which resorts systematically to the politics of the massacre' (pp. 168-169). These three different perspectives have a common denominator: we must consider this kind of violence not only as an ideological category, but as a form of extreme strategy. Extreme strategies such as this were 'morally justified' in different ways, varying widely between the perspective of total war and the perspective of a war against the Jews, or between the perspective of war against evil or the perspective of the logic of the lesser evil.

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