The aim of this cross‐sectional study was to assess the knowledge, attitudes and prevention practices (KAP) among the garment factory worker population in Bangladesh regarding a historical dengue outbreak in 2019. A total of 400 participants were selected by simple random sampling, and questionnaire‐based interviews were conducted. The average score of knowledge, attitude and prevention practice was 8.33 ± 2.35, 6.32 ± 1.20 and 6.31 ± 1.50, respectively. Only 76 out of 400 participants (19%) scored above 10 (all university‐educated). Participant workers reported both negative and positive attitudes regarding dengue fever (DF). Negative attitudes included an expectation of increased mortality and strained family finances from DF attacks. A significantly high number of participants (92%) believed that death from DF was inevitable. Positive attitudes included optimism about DF eradication potentials and eagerness to help and donate blood to sick relatives. Participants primarily learned about the DF prevention from mass media (244/400; 61.0%) and social media (97/400; 24.25%). The most popular prevention measures adopted were mosquito repellent incense (344/400; 86.0%) and mosquito nets (389/400; 97.25%). While most participants (358/400; 89.5%) cleaned areas where mosquitos lay eggs, only 169 out of 400 (42.25%) regularly treated with chemical sprays. Only 182 out of 400 (45.5%) reported receiving DF prevention training in the factory. Correlation between DF knowledge and education was statistically significant (r = .38, p < .01, n = 398). Correlation between DF knowledge and work experience was insignificant (r = .01, p > .01, n = 398). Age and DF knowledge were not correlated (r = 0.07, p > .01, n = 398). In conclusion, gaps in KAP for dengue could be addressed by government‐sponsored educational programmes that utilize the power of mass/social media for dengue prevention and control. More KAP surveillance studies are needed for other sectors of the society.

Canine parvovirus (CPV-2) is one of the most important pathogens of dogs of all ages, causing pandemic infections that are characterized by fatal hemorrhagic enteritis. The CPV-2 vaccine is recommended as a core vaccine for pet animals. Despite the intensive practice of active immunization, CPV-2 remains a global threat. In this study, a multi-epitope vaccine against CPV-2 was designed, targeting the highly conserved capsid protein (VP2) via in silico approaches. Several immunoinformatics methods, such as epitope screening, molecular docking, and simulation were used to design a potential vaccine construct. The partial protein sequences of the VP2 gene of CPV-2 and protein sequences retrieved from the NCBI were screened to predict highly antigenic proteins through antigenicity, trans-membrane-topology screening, an allergenicity assessment, and a toxicity analysis. Homologous VP2 protein sequences typically linked to the disease were identified using NCBI BLAST, in which four conserved regions were preferred. Overall, 10 epitopes, DPIGGKTGI, KEFDTDLKP, GTDPDDVQ, GGTNFGYIG, GTFYFDCKP, NRALGLPP, SGTPTN, LGLPPFLNSL, IGGKTG, and VPPVYPN, were selected from the conserved regions to design the vaccine construct. The molecular docking demonstrated the higher binding affinity of these epitopes with dog leukocyte antigen (DLA) molecules. The selected epitopes were linked with Salmonella enterica flagellin FliC adjuvants, along with the PADRE sequence, by GGS linkers to construct a vaccine candidate with 272 nucleotides. The codon adaptation and in silico cloning showed that the generated vaccine can be expressed by the E. coli strain, K12, and the sequence of the vaccine construct showed no similarities with dog protein. Our results suggest that the vaccine construct might be useful in preventing canine parvoviral enteritis (CPE) in dogs. Further in vitro and in vivo experiments are needed for the validation of the vaccine candidate.

Background: Approximately three thousand plastic goods manufacturing factories (PGMF) are currently operating in Bangladesh involving numerous workers. Associated health problems of these workers are largely unknown. The key objectives of the current study were identifying plastic chemical exposures related health outcomes in these workers and comparing these outcomes before and after their joining in PGMFs. In addition, we aimed to investigate the relationships between work duration and the prevalence of health ailments among workers. Method: A cross-sectional study was carried out among factory workers (n=405) at six PGMFs in Gazipur district in Bangladesh. A simple random sampling method had been applied to select participants and data on their self-reported exposures to chemicals and associated respiratory, neurological, and other multiple health outcomes were collected through a validated questionnaire survey. Data were analyzed using different descriptive and inferential statistical tools. The categorical variables and continuous variables were interpreted using frequency distribution and standard deviation (SD) respectively. A Pearson chi-square (χ2) test was applied to evaluate the correlation between work duration and health outcomes. A p-value <0.05 was considered significant statistically. Results: The average age and work duration of the workers were 25.63±6.85 and 3.49±3.53 years, respectively, implying that most workers were young, and spent over 10% of their lifetime in PGMFs work. Most common health outcomes reported by the workers were nasal discharges: 60 (14.9%), headaches: 76 (18.9%), fatigues: 112 (27.8%), losses of appetites: 108 (26.8%), urination problems: 61 (13.1%), losses of body weights: 102 (25.3%), and nervousness: 70 (17.4%). Among the common health outcomes only headache (p=0.005); fatigue (p=0.04); urination problem (p=<0.0001), and nervousness (p=0.004) were significantly associated with the work duration. Furthermore, except for hypertension and tarry stool, all health outcomes among workers differ significantly before and after joining in PGMFs. Conclusion: This study first time identified important health outcomes of the PGMFs workers and generated baseline information on common health outcomes of the PGMFs workers in developing countries like Bangladesh. However, it might be important to identify potential causes of such health outcomes in PGMFs workers considering both biomarkers of exposures and real-time environmental samples to understand the disease pathology and to recommend mitigation measures to be taken by occupational health policymakers and practitioners in developing countries.

Background: HIV prevalence in Bangladesh is low (<1%) among the general population, even within the vulnerable population it continued to be low other than certain sections of injecting drug users.Methods: This cross-sectional descriptive study was conducted in the Polly Chikitsok/rural medical practitioners in Hobiganj, Narayanganj, Rangpur and Kurigram District of Bangladesh during January 2014 to April 2014. One hundred eighty (180) Polly chikitsok/rural medical practitioners were interviewed with a semi-structured questionnaire. Purposive sampling technique was used. Statistical analysis of the results was done by using computer software SPSS version 22 with tabulation plan. The ethical committee of the department of public health, Daffodil International University of Bangladesh, approved the thesis with their signature.Results: A total of 180 participants were interviewed, of whom 176 were males and 4 were females. 17.8 percent of the respondents are of 21-30 years of age, 37 percent respondents are 31-40 years old. 27.8 percent of the study participants were in their 41-50. Hundred percent respondents, they had ever met any HIV positive patient in the course of their occupational responsibility in the workplace. 97.8 percent respondents correctly knew blood transfusions transmit HIV/AIDS, followed by sharing needles (98.3 percent), and maternal child transmission (68.9 percent). However, more than two thirds of the respondents (77.8 percent) correctly identified HIV/AIDS preventive measures. Nearly half the sampled population (84.4 percent) did not think condoms were useful in preventing transmission of HIV.Conclusions: In Bangladesh, the prevalence of HIV infection is growing and PC/RMPs are increasingly involved in a range of HIV prevention and care activities.