Synonymes Melibiase Galactosidase, .alpha . . alpha.-0-Galactosidase .alpha.-Galactosidase .alpha.-Galactosidase A .alpha.-0-Galactoside galactohydrolase . alpha.-Galactoside galactohydrolase CASReg. No.
9025-35-8
REACTION AND SPECIFICITY
Catalysed reactionAlpha-0-galactoside (galactose-ROH} + H 2 0 --->
3.2.1.22---> galactose + R (mechanism [1], hydro Iysis of terminal, non-reducing alpha-0-galactose residues in alpha-0-galactosides, including galactose oligosaccharides, galactomannans and galactolipids}
Reaction type
0-Giycosyl band hydrolysis, 57] 3 Melibiose + H 2 0 [1, 15, 33, 53] 4 Raffinose + H 2 0 [1, 2, 4, 15, 20, 29, 33, 44, 53) 5 Stachyose + H 2 0 [2, 15, 20, 29, 33) Enzyme Handbock ©Springer-Verlag Berlin Heldeiberg 1991 Duplication, reproduction and storage in data banks are only allowed with the prior permission of the publishers Alpha-galactosidase 3.2.1.22 6 Glycolipids + H 2 0 [40] 7 Galabiosylceramide + H20 [40]8 Globotriglycosylceramide + H 2 0 [40] 9 Disaccharides (and derivatives) + H 2 0 (overview) [5] 10 Trisaccharides (reducing and nonreducing) + H 2 0 (overview) [5] 11 Oligosaccharides + H 2 0 (overview) [5] 12 Polysaccharides+ H 2 0 (overview) [5] 13 More (galactosyl transferase activity [1,5,24,37,49,55], alphafucosidase activity [33], hydrolysis of alpha-D-galactopyranosidic linkages in glycosides of methanol, phenol and p-nitrophenol [5], Ieetin activity [33], not: substances with beta-galactosidic linkages [2], not: arabinosides [57]) [1, 2, 5, 24, 33, 37, 49, 55] Product spectrum 1 Galactose + ? (e.g. p-nitrophenol + D-galactose) 2 Fucose + ? (e.g. p-nitrophenol + D-fucose) 3 D-Galactose + D-glucose 4 Galactose + sucrose [2] 5 Galactose [2]
