No abstract

BackgroundNeonatal sepsis includes numerous systemic illnesses such as septicemia, meningitis, urinary tract infections, and pneumonia. In developing countries, the major reason for neonatal mortality is septicemia, which accounts for almost 50% of overall deaths. Thrombocytopenia is one of the most common hematological problems during the neonatal period, affecting the majority of sufferers admitted to the neonatal intensive care unit (NICU). The aim of our study was to find the frequency of thrombocytopenia and its severity in neonates with sepsis.

Objectives:This study’s aim was to find out that how various biochemical parameters of donor blood are affected during storage.Methods:This cross-sectional study was conducted in hematology Unit of Rehman Medical Institute, Peshawar and Fatimid Foundation, Peshawar Khyber Pakhtunkhwa, over a period of six months from June 2018 to November 2018. This study includes 300 healthy volunteer donors. Analysis of stored blood was done at 0, 3, 7, 14- and 21-days interval. Data were recorded and analyzed in SPSS v 20. A p value of less than 0.05 was taken as significant.Results:Three hundred healthy volunteer donors were included in the study in which 63% were male and 37% were females. Mean age was 26.54±7.3 years with age range from 23-46 years. Out of 300 donors 15.33% were O+, 35.33%, 9.66% B+, 8.33% A+, 7.66% AB+, 7.66% O-, 7% A- and 9% B-. Significant. Changes were observed in serum potassium, LDH, pH, serum chloride, serum sodium and AST levels. (p:<0.001). however, storage did not affect rest of the parameters.Conclusion:This study reveals that during storage certain changes occur in haematological and biochemical parameters which ultimately may put patients at risk.

Background: This study aimed to assess the frequency determination of c.1115_1118delTTGG and c.3788_3790delTCT Fanconi's anemia A gene (FANCA) gene mutation in the North of Khyber Pakhtunkhwa (KPK) Pakistan Fanconi’s Anemia Population. Materials and Methods: A cross-sectional study was conducted at Khyber Medical University, Peshawar, Pakistan. For the Exon 13 mutation c.1115_1118delTTGG, the amplification-refractory mutation system polymerase chain reaction (ARMS PCR) was developed. Sanger sequencing confirmed the ARMS PCR results for Exon 13 and found an exon 38 mutation of the FANCA (Fanconi anemia complementation group A) gene (c.3788_3790delTCT). Sanger sequencing results analyzed on Bio edit sequence aligner software confirmed the results of PCR. The four incidental single nucleotide polymorphisms (SNPs) discovered were examined in several variation databases. Results:  The mean age for the patients was 9.68±3.02 years, with an age range of 5–16 years. Pedigree analysis of Fanconi Anemia patients revealed an autosomal recessive pattern of inheritance. Physical characteristics such as skeletal abnormalities, specifically thumb abnormalities and Fanconi's facies, are distinctive diagnostic features of FA. Pedigrees also showed bone marrow hypoplasia in 65% of patients and red cell aplasia in 6%. PCR results from all samples revealed tahat none of them had the Exon 13 mutation. In addition, none of the samples had the Exon 38 mutation. Four SNPs were found in the Sanger sequencing. Two of them were in the intron 12 region, one in each of Exons 13 and 38. Conclusion: Results show that mutations in Exon 13 and Exon 38 of the FANCA gene are uncommon in our Pakistani FA population. SNPs established in the Pakistani population of Khyber Pakhtunkhwa (KPK) province have not been reported before.