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This study aimed to investigate the effects of two, conventional and green, extraction techniques on the nutritional, bioactive and physicochemical properties of oils from Astrocaryum vulgare pulp. It is an Amazonian oilseed named tucumã, known by their oily nature and bioactive properties. n-Hexane (N-HE) and supercritical CO 2 (SFE-CO 2 ) were used as extraction solvents. Higher oil yield, 39.63%, was achieved using N-HE then SFE-CO 2 , 36.75%. The SFE-CO 2 oil presented low acidity and peroxide content, 0.9 mg KOHg −1 and 1.1 mEq kg −1 , while the N-HE presented 3.25 mg KOH g −1 and 2.4 mEq kg −1 , respectively (P < 0.05). The SFE-CO 2 oil exhibited greater content of linoleic (~42.3%) and oleic acids (~32.6%) (P < 0.05) and higher β-carotenes, up to 1100 μg 100 g −1 , while the N-HE oil had ~877 μg 100 g −1 . SFE-CO 2 had positive effect on the physicochemical and nutritional properties of A. vulgare pulp oil, which was statistically confirmed by principal component analysis (PCA). Practical applications The application of green techniques based on supercritical CO 2 fluid extraction to obtain biolipids from A. vulgare pulp with greater nutritional quality contributes to its potential utilisation as a functional ingredient in foods.

The use of clean technologies in the development of bioactive plant extracts has been encouraged, but it is necessary to verify the cytotoxicity and cytoprotection for food and pharmaceutical applications. Therefore, the objective of this work was to obtain the experimental data of the supercritical sequential extraction of murici pulp, to determine the main bioactive compounds obtained and to evaluate the possible cytotoxicity and cytoprotection of the extracts in models of HepG2 cells treated with H2O2. The murici pulp was subjected to sequential extraction with supercritical CO2 and CO2+ethanol, at 343.15 K, and 22, 32, and 49 MPa. Higher extraction yields were obtained at 49 MPa. The oil presented lutein (224.77 µg/g), oleic, palmitic, and linoleic, as the main fatty acids, and POLi (17.63%), POO (15.84%), PPO (13.63%), and LiOO (10.26%), as the main triglycerides. The ethanolic extract presented lutein (242.16 µg/g), phenolic compounds (20.63 mg GAE/g), and flavonoids (0.65 mg QE/g). The ethanolic extract showed greater antioxidant activity (122.61 and 17.14 µmol TE/g) than oil (43.48 and 6.04 µmol TE/g). Both extracts did not show cytotoxicity and only murici oil showed a cytoprotective effect. Despite this, the results qualify both extracts for food/pharmaceutical applications.