Resumo No presente estudo foram avaliadas as soropositividades para Leptospira interrogans e Brucella abortus, através das provas de soroaglutinação microscópica e aglutinação em tubo, respectivamente, em pacientes da área rural que procuraram o Posto de Saúde do município de Guaraci, Paraná, Brasil. Das 115 amostras de soros testadas, três (2,6%) apresentaram-se sororeagentes para Leptospira spp. Com relação ao estudo sorológico da brucelose não foram verificados títulos positivos. Palavras-chaves: Leptospirose. Brucelose. Prevalência. Leptospira interrogans. Brucella spp.
AbstractThe present study evaluated patients that were attended at the Health Center of Guaraci Municipality, Paraná State, Brazil. Three (2.6%) patients were leptospirosis positive from a total of 115 samples. No significant statistical differences were observed for risk factors. There was no positive test for brucellosis. Key-words: leptospirosis. brucellosis. Prevalence. Leptospira interrogans. Brucella spp. Verificou-se que das 115 amostras de soros testadas, três (2,6%) foram soropositivos e 112 (97,4%) foram soronegativos para leptospirose. Os títulos observados foram 100 (casos n° 12 e 33) e 400 (casos n° 95), para o sorovar icterohaemorrhagiae e 100 (caso n° 95) e 200 (caso n° 33) para o sorovar autunamlis.Através dos dados epidemiológicos, verificou-se uma diferença significativa em relação aos pacientes que relataram ter auxiliado partos de animais, conforme
SUMMARYAn autochthonous case of visceral leishmaniasis is reported in a dog (Canis familiaris) as an apparently natural infection in a nonendemic area. DNA obtained from spleen and liver samples produced the expected fragment in a Leishmania-specific rDNA-based nested-PCR assay. The PCR product, a 490 bp fragment, was sequenced and the nucleotide sequence was identical to that of Leishmania (Leishmania) infantum chagasi. These results are surprising since no autochthonous human or canine cases of visceral leishmaniasis have ever been reported in this municipality. This case suggests that natural transmission of this disease is occurring in this area.
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