Although Trachops cirrhosus (Spix, 1823) is known for feeding on frogs, most of the data on its feeding behavior comes from the analysis of stomach contents, remains on roosting sites or experimental feeding in captivity, with few cases observed directly in the field. Here we report T. cirrhosus preying upon the frog Leptodactylus macrosternum Miranda-Ribeiro, 1926, which is the first record of this bat species preying an anuran in southeastern Brazil, and also the first direct observation of a predation event by T. cirrhosus in the wild in this region, contributing to the understanding of its trophic relations.