No abstract

No abstract

The aim of this study was to evaluate changes in polyphenol profile and antioxidant capacity of five soluble coffees throughout a simulated gastro-intestinal digestion, including absorption through a dialysis membrane. Our results demonstrate that both polyphenol content and antioxidant capacity were characteristic for each type of studied coffee, showing a drop after dialysis. Twenty-seven compounds were identified in coffee by HPLC-MS, while only 14 of them were found after dialysis. Green+roasted coffee blend and chicory+coffee blend showed the highest and lowest content of polyphenols and antioxidant capacity before in vitro digestion and after dialysis, respectively. Canonical correlation analysis showed significant correlation between the antioxidant capacity and the polyphenol profile before digestion and after dialysis. Furthermore, boosted regression trees analysis (BRT) showed that only four polyphenol compounds (5-p-coumaroylquinic acid, quinic acid, coumaroyl tryptophan conjugated, and 5-O-caffeoylquinic acid) appear to be the most relevant to explain the antioxidant capacity after dialysis, these compounds being the most bioaccessible after dialysis. To our knowledge, this is the first report matching the antioxidant capacity of foods with the polyphenol profile by BRT, which opens an interesting method of analysis for future reports on the antioxidant capacity of foods.

Pseudofruits of Hovenia dulcis, at five different ripening stages, were evaluated in order to characterize their bioactive compounds (phenolic compounds, tocopherols and fatty acids), and determine their biofunctionality (antitumor, antimicrobial and antioxidant activity). Epi(gallochatechin) was the main phenolic compound (24.7 mg/g, Hd05), a-tocopherol was the main isoform, increasing its content with the maturity process (5.43 mg/100 g, Hd04), C18:2 was the main polyunsaturated fatty acid (35.5%, Hd04), while C16:0 was the predominant saturated fatty acids (38%, Hd01). The most immature stages (Hd01-Hd02) showed antitumor activity against all tested tumor cell lines, mainly against NCI-H460; also Hd01 and Hd02 presented the highest antimicrobial activity, mainly against Pseudomonas aeruginosa and Staphylococcus aureus. This underutilized product demonstrated to be a rich source of phenolic compounds and tocopherols showing an interesting activity in immature stages (unfit for consumption in natura) and could be use as alternative bioactive ingredient for functional foods, dietary supplements or nutraceuticals.

No abstract