Microorganisms are only effective when adequate conditions for their survival and development are provided. Among the factors that influence its effectiveness includes the type of soil or culture substrate, which works as an energy source reserve. Therefore, a tomato and a melon crop were established in different cycles to assess the effect of the physicochemical properties of organic substrates based on coconut fibre and vermicompost in three proportions, 0:100, 40:60 and 60:40 (% v:v), on the microbial activity in the rhizosphere when the bacteria Azotobacter vinelandii, Bacillus megaterium and Frateuria aurantia were applied. Concentrations of NO3−, H2PO4−, K+ and Ca2+ in the petiole cellular extract (PCE) were quantified at 60, 90 and 120 days after transplantation (DAT) for tomato and 45 and 65 DAT for melon. We analysed dehydrogenase activity (DHA), acid phosphatase activity (FTA) and β-glucosidase activity (β-GLU). In order to maintain optimal volumetric moisture for the survival of microorganisms, automatic control was used to manage the irrigation frequency between 22%–28%. The results showed that physicochemical substrate properties, by incorporating 40% vermicompost into the coconut fibre mixture, increased enzymatic activity. Plants that were inoculated with Azotobacter vinelandii and Frateuria aurantia showed an improvement in NO3− and K+ assimilation achieving highest yields.
Saffron is traditionally cultivated in soil as a semi-perennial crop, although the feasibility of crop production is today constrained in Europe due to both agronomic and socioeconomic factors. Accordingly, interest has been increasing concerning its possible cultivation within protected environments through adoption of soilless cultivation technologies. The aim of the present study was to optimize nutrient solution features in the soilless cultivation of saffron corms. The trial was conducted in a greenhouse at Almeria University. Saffron was grown in 15-L pots filled with perlite. Three fertigation treatments were used, obtained by a linear increase of all nutrients of one standard in order to reach an electrical conductivity (EC) of 2.0 (control, EC2.0), 2.5 (EC2.5) and 3.0 (EC3.0) dS·m−1. Measurements included determinations of shoot length, corm yield, as well as nutrient uptake from the nutrient solution and concentrations within plant tissues. The nutrient solution with the highest EC (EC3.0) allowed obtaining three to five times more corms above 25-mm diameter. The increasing EC had a significant effect on the increase of macronutrient uptake, except for NO3− and NH4+ and resulted in a general increase of nutrient concentrations in tissues, such as corms and roots. Both macronutrient uptake and accumulation in plant tissues were highest under EC3.0. Nutrient uptake was significantly correlated with production of larger corms due to higher horizontal diameter.
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