The armadillo repeat super-family proteins (ARM repeat super-family proteins) possess tandem armadillo repeats and have been postulated to play different roles in plant development, morphogenesis, defense, cell death, and signal transduction through hormone signalling. In The Arabidopsis Information Resource (TAIR), we found 113 loci closely related to ARM repeat family proteins. This extensive group of proteins was studied in flowers tissues by western blot using antibodies directed against the most conserved region of the ARM repeat family proteins. The amino acid residues sequences from TAIR were aligned and the resulting phylogenetic tree allows us to inferring their evolutionary relationships. The main finding was the high similarity between the gene product of PUB16 (At5g01830, <i>A. thaliana</i>) and ARC1 (<i>Brassica napus</i>). In order to search a possible role for PUB16 we carried out stress bioassays using hormonal and saline approaches. Gene expression using RT-PCR showed that some of the ARM repeat super-family proteins are expressed both under salt or hormonal stress conditions. Particularly these studies allowed to detect and semi-quantify PUB16 gene expression in normal or stress growth conditions. In this approach it was revealed that, only in presence of GA, the expression of mRNA-PUB16 became evident. To morphologically verify the increasing number of germinated pollen grain in gibberellins treated flowers, we used epi-fluorescence microscopy assay. These results suggest that PUB16 may participate in GA signaling pathway favoring self-pollination
Five known compounds were isolated from the aerial parts of Colliguaja integerrima Gillies & Hook (Euphorbiaceae). A pharmacological evaluation of the diuretic activity of the plant was carried out in isotonic saline-loaded rats. The data reported in the present work indicate that the aqueous extract of the aerial parts from C. integerrima showed a moderate diuretic activity. The urinary levels of sodium and potassium are increased by treatment with C. integerima aqueous extract. This activity could be due, in part, to the presence of betulin isolated as the major metabolite of C. integerrima.
Los delitos informáticos, son actos ilícitos cometidos mediante el uso inadecuado de la tecnología, atentando contra la privacidad de la información de terceras personas, dañando o extrayendo cualquier tipo de datos que se encuentren almacenados en servidores o gadgets. El objetivo de la investigación es determinar los principales tipos de delitos informáticos existentes y, los riesgos que estos generan para la sociedad, las empresas y los gobiernos. Es una investigación de tipo mixta, apoyados en estudios confirmatorios y estudios exploratorios, resaltando teorías y Leyes que tienen relación con el estudio. Los resultados de la investigación, arrojan la importancia de determinar los diferentes tipos de delitos informáticos existentes. Así como, el reconocimiento de los puntos álgidos respecto a la seguridad de las redes, para contrarrestar su expansión. Finalmente, los delitos informáticos representan toda acción anti-jurídica, vía cibernética,
The effect of previous exposure on discrimination of two flavored solutions sharing a common taste was investigated in a pilot experiment. These solutions were at the limit of discrimination and the pre-exposure and test phases took place on different days. The results showed that mere pre-exposure (i.e., in absence of feedback) facilitates subsequent discrimination of same or different pairs of solutions. This result is in some aspects opposite to those reported recently by Dwyer and colleagues. This discrepancy is discussed analyzing the procedural differences observed between these studies.
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