ClinicalTrials.gov: NCT00893711.

Aim: This study compared the faecal microbial composition of formula-fed infants who did and did not have colic.Methods: Faecal samples from formula-fed infants under 16 weeks of age with (n = 38) and without (n = 39) colic were collected at Department of Pediatrics in Turin, Italy, between February 2014 and October 2015. The pH and faecal ammonia were determined and total bacteria, bifidobacteria, lactic acid bacteria and coliforms were quantified by fluorescent in situ hybridisation (FISH).Results: Faecal ammonia was significantly higher in the colicky infants than in the controls (483 vs. 216 lg/g, p < 0.05). The FISH counts of total bacteria were lower in colicky infants (1.8E10 AE 1.5E10) than in the controls (3.4E10 AE 3.0E10) (p < 0.05). The relative abundance of coliform bacteria was significantly higher in colicky infants (p < 0.05). No differences were observed for the bifidobacteria and lactic acid bacteria counts between the two groups.Conclusion: Our comparison of formula-fed infants with and without colic revealed significant differences in total bacteria, Enterobacteriaceae and faecal ammonia. This study provides the stimulus for further studies of the gut microbiome, using new methods of analysis such as 16S metagenomics sequencing in order to lead to more tailored dietary approaches.

Regulatory T cells induce immune homeostasis and the expression of Toll like receptors (TLRs); subsequent inflammatory cytokine release may be involved. Recent studies have shown a microbial imbalance in the gut of colicky infants (with a prevalence of gram-negative bacteria, such as Escherichia coli), and accumulating evidence has shown the efficacy of a probiotic (Lactobacillus reuteri) in breastfed subjects, but the underlying mechanism remains undefined. The study enrolled 59 infants younger than 60 days, of whom 34 subjects had colic and 25 were healthy controls. With a double-blind, placebo-controlled randomised study performed in our unit from October 2016 to July 2017, infants with colic were randomly assigned to receive oral daily L. reuteri DSM17938 (1×108 cfu) or placebo for 28 days. Peripheral blood was collected to assess the expression of FoxP3, TLR2 and TLR4 mRNA using real-time TaqMan RT-PCR at baseline and after the study period. Our findings showed increased mRNA expression of the transcription factor forkhead box P3 (FoxP3) in infants treated with L. reuteri DSM 17938 for 28 days (P<0.009) and increased TLR2 and TLR4 mRNA expression in both treated and placebo subjects. After L. reuteri administration for 28 days in infants with colic, we observed a significant decrease in daily crying time (302.3±19.86 min/day on day 0 vs 76.75±22.15 min/day on day 28, P=0.001). This study provides evidence that the observed increase in FoxP3 expression and reduction in crying time might be responses to probiotic treatment, while the increase in TLR2 and TLR4 mRNA expression might be related to age. Exploiting these new findings may lead to an unprecedented level of therapeutic control over immune tolerance using probiotics.

BackgroundPrevious research demonstrated several benefits of strategic perspective taking in the field of intergroup relations and, more specifically, in the negotiation processes aimed at conflict resolution. The present study, which analyzes the effect of perspective taking and mediation in a conflict setting, corroborates the psychological models that hypothesize the positive effects of the assumption of the competitor’s perspective on having intergroup conflict and lessening of negative consequences.Materials and methodsAfter being involved in an epistolary debate on a topic for which their ingroup had very contrasting views compared to an outgroup, participants were asked to use a feeling thermometer to assess their level of intergroup hostility. Mediation was then used as a conflict-resolution strategy for half of the participants. Furthermore, the assumption of perspective was manipulated, resulting in a 2 (conflict: presence vs absence) × 2 (perspective taking: presence vs absence) × 2 (mediation: presence vs absence) between-subjects design. Finally, participants were asked to use the same feeling thermometer to evaluate whether feelings of hostility diminished.ResultsThe results show that in conflict situations, the level of hostility decreases the most when the mediation process is accompanied by perspective taking.ConclusionThe results extend recent results about the advantages of a significant social ability – perspective taking – for improving intergroup relations.

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