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The number of women on military missions has been increasing recently. While on military missions, they live in an isolated group dominated by men. Such groups consist of young, sexually active individuals, thus increasing the risk of sexually transmitted infections.Medical examinations were performed on various residual forces (military, police, security guards) totaling 579 individuals, up to 35 years of age (172 women and 407 men). Subjects qualifi ed for the study were divided into 2 groups. Group I consisted of persons who remained in the country. Group II included persons stationed on military missions abroad. A total of 306 soldiers from this group participated in Study 1. Study 2 (clinical and laboratory) was carried out 6-12 months later on 52 patients from Group II and 119 patients from Group I (84 women and 87 men). Additionally, transcription of HPV genes E6 and E7 (with high oncogenic risk) was carried out in the group made up of 200 women. Laboratory examinations included: peripheral blood morphology, lymphocyte subpopulations by fl ow cytometry method (CD45, CD3, CD19, CD4, CD8 markers and NK cells CD16+CD56 markers), IL-2, IL-4, TNF-α, IFN-γ, IL-18 concentration by fl ow cytometry method. IgG, IgA, IgM concentration by turbidimetric method and

(Suppl 1):A1Background Asthma is the most common chronic respiratory disease in childhood. However, it is quite difficult to make a prompt diagnosis of asthma in a child 5 years and younger, partly due to a lack of objective diagnostic means. Our previous studies on susceptibility gene of asthma showed that a gene-gene interaction among 4 single nucleotide polymorphisms including IL13 rs20541 and IL4 rs2243250, ADRB2 rs1042713 and FcER1B rs569108 had a predictable role for asthma in wheezing children. Objective The study was aimed to further investigate the predictive effects of the four-loci interaction model in Chinese preschool children with asthma. Methods A total of 212 wheezing children aged 6 months to 5 years were enrolled and followed up for at least one year at Shanghai Xinhua Hospital between Dec 2014 and Mar 2016. Clinical data and lab findings of atopy were collected. All the children were divided into the high-risk group and the low-risk group according to genotypes of the four-loci interaction model. The differences of clinical features were compared between the two groups. The predictive effects for asthma were analyzed among asthma predictive index (API), 2015 Canadian Diagnostic Criteria for Asthma in Preschoolers and our four-loci interaction model. Results Of all the enrolled 212 children, 117 (55.2%) were assigned into the high-risk group and 95 (44.8%) were the low-risk group. Compared with the low-risk group, the high-risk group had more yearly episodes of wheeze and a higher level of blood eosinophilia. More children in the high-risk group presented with afebrile wheeze, eczema and positive food or aero allergens and had a history of tobacco exposure. If the asthma-predictive effect of positive API was considered as 1, the four-loci interaction model had a sensitivity of 77.2%, a specificity of 80.0% and an AUC area of 0.786 with a modest consistency (P=0.22, Kappa=0.49), while the Canadian criteria had a sensitivity of 97.8%, a specificity of 53.3% and an AUC area of 0.539 with a low consistency (P<0.01, Kappa=0.265). ConclusionsThe four-loci interaction model is associated with the phenotypes of wheezing in Chinese preschoolers. It has a consistent predictive effect with API for asthma and is more specific than the Canadian criteria in the diagnosis of asthma, which indicates that the four-loci interaction model may be developed as a new objective predictive tool for asthma in Chinese children 5 years and younger. A2Loss of esophageal epithelial SPINK7 unleashes uncontrolled proteolytic activity, impaired epithelial barrier, defective differentiation and pro-inflammatory cytokine production Background Epithelial barrier impairment has been implicated in the development of allergic disease. However, the molecular mechanisms by which impaired epithelial barrier function induces Th2-type immune responses remain largely unknown. In this study, we examined the role of the serine peptidase inhibitor kazal type (SPINK)7 on epithelial barrier function and mucosal Th2-associated immune responses...

BackgroundInfections caused by various microbes may induce immunodeficiency and allergies.MethodsTests were conducted on 579 potentially healthy people (172 women and 407 men, between 25 and 30 years), performed twice: in a diagnosis and after 6 to 12 months. Lymphocytes and NK cells from peripheral blood were assessed using cytometry. G, A, M immunoglobulin concentrations were determined by the turbidimetry. IgE concentration was measured by ELFA. HSV2, HIV and Chlamydia trachomatis infections were detected on the basis of presence of IgM and IgG antibodies tested by ELISA. For the assessment of HPV infections, DNA from the urinary-tract, squamous, epithelial cells were tested through PCR. The statistical analysis was undertaken using regression analyses.ResultsIn 579 people in the case of 65 people HPV infections were confirmed 10% men, 14.8% women. In 65 patients, in 1 case concentration of IgA was decreased, in 7 cases IgM. In 21 cases, there was higher concentration of IgE. During the cytometric analyses a decreased number of T CD3+ was found in 3 cases, BCD19+ in 5, TCD4+ in 5, T CD8+ in 4, and NK cells in 8 cases. The check for HSV2 was positive for 17 people: 1.5% men, 6.4% women. In this group, in 3 cases the concentration of IgA was decreased, in 2 cases IgM was lowered, in 4 cases there were higher concentrations of IgE. Decreased number of lymphocytes CD19+ was observed for 1 person and for 1 person NK cells. Chlamydia trachomatis infections were positive in 10 cases: 1.5% men, 2.3% women, in this group 1 person had lower IgA and 2 persons had increased concentrations of IgE. HIV infection was negative for all the groups. The examination was repeated for 171 out of 579 people after 6 months. HPV infections were positive in 12.9% cases: 14.9% men, 10.8% women; HSV2 in 11 persons: 2.3% men, 10.7% women; Chlamydia trachomatis in 4 cases: 2.3% men 2.4% women.ConclusionsNo significant correlation was observed between HPV, HSV2, HIV and Chlamydia trachomatis infections and a relevant deviation from the norm of the investigated immunological parameters.

BackgroundThe type I or IV of hypersensitivity reactions according to Gell and Coombs classification may be responsible for clinical symptoms observed after ingestion of gluten - containing products. The mechanisms of these reactions are either IgE-dependent or IgE-independent. Celiac disease based on IgE-independent mechanism is classified as gluten hypersensitivity. Clinical manifestation of celiac disease and gluten allergy is often similar. Correct diagnosis of this disease is particularly important due to the different long-term therapeutic procedures. We would like to asses of the incidence of celiac disease in children with gluten allergy.MethodsThe study involved 50 children with abdominal pain, chronic diarrhea, recurrent respiratory and ears inflammation and skin lesions - patients of the Immunological and Gastroenterology Outpatient Clinic of Institute of Mother and Child. The allergy to gluten was confirmed on the basis of positive peripheral blood lymphocytes blast transformation test and detection of allergen-specific IgE antibodies to gluten (f79). In all children plasma concentration of immunoglobulin classes A, G M and IgA or IgG antibodies against tissue transglutaminase (tTGA) were measured.ResultsIn children on the study group the type IV of hypersensitivity reaction to gluten was diagnosed. In 3 children specific IgE antibodies to gluten was also confirmed (f79 - I type hypersensitivity). Anti-tissue transglutaminase antibodies both IgA and IgG were detected in 2 children in whom the concentration of IgA and IgG in serum remained within normal range for age. In these children celiac disease was confirmed by jejunal biopsy.ConclusionsThe predominant frequency of type IV of hypersensitivity reactions in children in response to the gluten antigen should be taken into account in diagnosis of food allergy.In children diagnosed with gluten allergy the test for celiac disease should be performed.