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A C-terminal region of human endothelial actin-binding protein-280 (ABP-280 or ABP, non-muscle filamin) was subcloned and efficiently expressed in a mammalian cells system as indicated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting analysis. As predicted by the aminoacid sequence, the fragment, a 79 kD peptide (residues 1671-2361, plus 3.9 kD from an N-terminal fusion peptide included in the expression plasmid), contained the two potential cAMP-dependent protein kinase (PKA) phosphorylation sites (serine 2152 and threonine 2336) predicted to be present in this region of the molecule. Incubation of cells in the presence of cAMP-elevating agents enhanced 32P uptake into the fragment. Site-directed mutagenesis analysis indicated that serine 2152 is the unique substrate in the C-terminal region of ABP for endogenously activated PKA. The functional implications of phosphorylation of this residue, which belongs to a serine-proline motif, are discussed in terms of the role of filamin in cytoskeleton reorganization.

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In this paper, I examine how close care relations, sometimes resulting in kinship, are made between Mexican immigrant women employed as private care providers by aging Anglo‐Americans. This study is based on ethnographic fieldwork in Santa Barbara, California, between 2009 and 2011 and focuses on the informal care market. I discuss three case studies, applying insights from the new kinship literature, and I focus on one extended case to show how it reflects and challenges traditional notions of family in the United States. At a time when questions about what constitutes appropriate intimacy in paid care are rife, studies such as this are crucial to better understand the contexts in which other forms of kin relations may be created and solidified.