Campylobacter jejuni subsp. jejuni is one of the most frequent causes of infectious diarrhea in both, developed and developing countries (Fernández 1992, Allos & Blaser 1995. Although C. jejuni subsp. jejuni diarrhea is a self-limited disease, in some clinical instances it is necessary the prescription of antibiotics, being erythromycin the drug of choice (Allos & Blaser 1995). For several years however, higher levels of resistance to this drug, as well as to others, such as tetracycline and fluoroquinolones, have been reported in several countries (Taylor & Courvalin 1988).The information on bacteriological, biological, pathogenical, clinical and epidemiological aspects of C. jejuni subsp. jejuni generated in South America is vast and valuable (Fernández 1992). However, the susceptibility patterns of this bacterium have not been well defined in this geographical region.The aim of this study was to determine the susceptibility patterns of C. jejuni subsp. jejuni clinical strains, to six antimicrobial agents, in Southern Chile by using two quantitative methods.A total of 108 C. jejuni subsp. jejuni strains submitted in this study, had been isolated and identified from pediatric diarrheic stools specimens using standard procedures between March 1996 and December 1997. Strains were stored at -35ºC in freezing medium until analyzed. Staphylococcus aureus ATCC 25923 and Escherichia coli and E. coli ATCC 25922 were used as control strains.The antimicrobial susceptibility patterns of the strains under study were determined by means of the E-test and the double dilution agar method (Baker et al. 1991).To perform the E-test, several colonies of each strain, obtained from a fresh culture in blood agar plate, were suspended in 5 ml of Mueller-Hinton broth to achieve a turbidity equal to the 0.5 Mac Farland standard. The suspensions were inoculated with sterile swabs onto 150 mm diameter MuellerHinton 5% sheep blood agar plates and after the agar surfaces were allowed to dry, six E-test strips were applied on each plate. Plates were incubated at 37ºC for 48 h under microaerobic conditions and inhibitory concentrations were read at the point where the elliptical zone of inhibition intersected the E-test strip.For the agar dilution method, Mueller-Hinton agar plates containing serial twofold dilutions of each antimicrobial agent from 0.125 to 128 µg/ ml were prepared. Inocula of the strains were prepared diluting 1:10 each bacterial suspension made in Mueller-Hinton broth with a turbidity equal to the 0.5 Mac Farland standard. Plates were inoculated with a multipoint replicator delivering spots of 4This work received financial support from grants S-97-21 DID-UACH and FONDECYT 1980920. + Corresponding
Resumen La Terapia Ocupacional es una disciplina emergente dentro de los equipos educativos multidisciplinarios, por lo que, resulta relevante indagar sobre su rol dentro del contexto educativo chileno. Este artículo presenta una investigación cualitativa cuyo objetivo es analizar desde una perspectiva crítica, cómo influye el diagnóstico clínico en las prácticas de terapeutas ocupacionales que trabajan en contextos educativos, considerando como ejes principales para la investigación, el marco normativo inclusivo vigente en Chile, el discurso de terapeutas ocupacionales a cerca de sus prácticas y las relaciones que se establecen con el diagnóstico clínico en este contexto. La muestra corresponde a diez terapeutas ocupacionales que se desempeñan en establecimientos educativos del sur de Chile, a quienes se les aplicaron entrevistas semi-estructuradas. Los resultados permiten visualizar que las políticas educativas influyen significativamente en las prácticas de terapeutas ocupacionales, aún cuando la normativa no especifica el rol disciplinar. Además, el diagnóstico circula fuertemente en el discurso de terapeutas ocupacionales, funcionando como un dispositivo de poder al interior de las prácticas profesionales, especialmente con el fin de validar el rol profesional dentro del contexto educativo. Se discute el uso del diagnóstico en relación a la identidad profesional y los efectos que tiene sobre los sujetos de intervención. El artículo concluye con la propuesta de adoptar una visión crítica del propio quehacer de la disciplina, basándose en la acción transformadora que ésta busca generar a través de la práctica.
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