The present study aimed to evaluate the effectiveness of an amaranth-based beverage (CHO-P) on cycling performance and hydration status, despite containing a total caloric content higher than that of a commercial sports beverage (CHO-P: 52.48 kcal per 100 mL vs CHO: 24 kcal per 100 mL). In a randomized, crossover design, six cyclists performed two exercise tests separated by seven days. Each test comprised two time-trials (32.20 km and 5 km) separated by 10 min of rest. Participants consumed either an amaranth-based beverage (CHO-P; 10% and 1.5% concentrations) or a commercial sports beverage (CHO; 6%). Changes in hematocrit and body mass, ratings of perceived exertion, and average power were assessed throughout both tests. 32.2-km time-trial performance was enhanced with CHO-P compared to CHO (54.3 ± 4.1 min vs 55.6 ± 4.8 min; p<0.05). However, no other variablemeasured in this study was significantly different between beverage types. Further laboratory based research should be performed to further explore the ergogenic potential of amaranth supplementation during endurance exercise.