Results of in vivo optical immersion clearing of human skin under the effect of aqueous solutions of a number of immersion agents (monosaccharides of ribose, glucose and fructose, as well as glycerol, a triatomic alcohol) were obtained with the use of the OCT method. Values of average velocity of scattering coefficient change, which were obtained through an averaged A-scan of the OCT signal in a derma region with the depth from 350 to 700 µm, were determined in order to evaluate the optical clearing efficiency. Good correlation was identified between the velocity of scattering coefficient change and the optical clearing potential value. Molecular modeling of interaction of a number of immersion clearing agents with collagen allowed to identify correlations between the optical clearing efficiency and the energy of intermolecular interaction.