No abstract

Different types of sealing and sucrose concentrations influence in vitro elongation and adventitious rooting. Higher gas exchange (CO 2) favors the in vitro plant growth. The autotrophic system for the in vitro cultivation of Eucalyptus dunnii was not efficient.

Spectral quality is an important factor for in vitro development of explants in a bioreactor system. Based on the need to optimize micropropagation for E. grandis × E. urophylla clones, the aim of the study was to assess the spectral quality of in vitro multiplication in temporary immersion bioreactor (TIB). The tissue used to generate the explants (i.e., the nodal segment with 1 cm of length and two axillary bud without leaves) was previously in vitro established and multiplied, it derived from ministumps of E. grandis × E. urophylla clone grown in a semi-hydroponic system. The spectral quality of in vitro multiplication was assessed through five light sources (i.e., fluorescent lamp, red, green, blue, and yellow cellophane). Morphological and anatomical features of tissues grown in TIB were evaluated at 90 days. Based on the results, yellow and blue spectral qualities were the most suitable to be adopted for in vitro multiplication of E. grandis × E. urophylla, since they enabled lesser hyperhydricity, favors high number of shoots per explant and shoot length, as well as thicker mesophyll and spongy parenchyma; arise as an alternative for large-scale production of eucalypts clonal plants.

The importance of Eucalyptus and Corymbia has been evident in forestry programs, mainly due to their adaptation to various environmental conditions and the multiple products that can be obtained. As the selection and cloning of superior individuals are usually performed in adulthood, the development and adaptation of the vegetative propagation techniques that enable the rescue and in vitro establishment of species are necessary. Thus, the present study aimed to induce epicormic shoots from pruned branches of adult trees and to promote the in vitro establishment of nine species: six of Eucalyptus and three of Corymbia. The material used to obtain the explants came from the selection of two 44-year-old mother plants of each species, from species, and provenance tests. The number of buds and shoots of each branch was evaluated at 15, 25, 35, and 45 days after the beginning of the experiment in a greenhouse. According to the results, the induction of epicormic shoots from pruned branches was considered a viable and efficient technique to obtain propagule sources from adult mother plants of all species, presenting a gradual increase in the evaluated characteristics over time. Also, in vitro culture is an effective alternative to improve the propagation of the evaluated species, with an establishment of up to 62.5%. However, for Corymbia citriodora (Hook.) K.D. Hill & L.A.S Johnson and Eucalyptus tereticornis Smith it is necessary to develop further studies to enable the in vitro establishment.

MacLeish is economically appreciated due to its wood and to the extraction of its essential oil, whose active principle is alpha-bisabolol. Despite its economic importance, studies about the genetic breeding of this species remain scarce. The aim of the current study is to determine E. erythropappus seedling emergence rate, genetic parameters, as well as to identify potential open-pollinated progenies of this species and their respective matrices. The experiment was set up in tree nursery, based on a completely randomized design, with 22 progenies in four replicates comprising 14 plants per plot. Seedling emergence rate was evaluated 30 days after seeding, whereas plant height (H) and stem diameter (SD) were measured at 100 days. Statistical analyses were carried out based on the REML/BLUP method. Three progenies have shown seedling emergence rate lower than 50%; this value is considered limiting to seedling production process. Genetic variation was observed among progenies, as well as high and positive genetic correlation between H and SD. Thus, it was possible identifying superior progenies and matrices, as well as the ones presenting low performance in the evaluated traits.