All over the world, fermented dairy products have been consumed for nutrition and maintenance of good health for a very long time. This study evaluated the survival of Lactobacillus delbrueckii ssp. bulgaricus and Bifidobacterium animalis ssp. lactis BB-12 in yoghurts after the manufacturing during the shelf-life up to 21 days at 4 °C, which is mostly accepted by the consumers. The titratable acidity and pH showed the same patterns of increase or decline after manufacturing and storage of yoghurts. There was a significant difference (p <0.05) in acidity between yoghurts in glass bottle and plastic cup. The increase in numbers of lactobacilli and bifidobacteria and their survival during storage time were dependent on the species and strain of associative yoghurt bacteria (control-only yoghurt lactic acid bacteria and experimental containing except yoghurt culture also Bifidobacterium animalis ssp. lactis BB-12) and on the packaging material (glass bottle versus plastic cup). It was observed, that counts of bifidobacteria were lower than counts of Lactobacillus delbrueckii ssp. bulgaricus (190 to 434 x 10 7 at 1d) and slowly increased (p <0.001) at maximum level on day 7 (294.3 -754 x 10 7 ) and then slowly declined to 6.33 x 10 7 in glass bottle and 2.33 x 10 7 in plastic cups, respectively. Lactobacillus delbrueckii ssp. bulgaricus multiplied better in glass bottles than in plastic cups, as observed during experimental period in-group with Bifidobacterium animalis ssp. lactis BB-12. At the end of the storage period at 4 ºC, viable counts of lactobacilli were higher (p <0.001) in glass bottles. Al the yoghurts, contained the recommended levels of lactobacilli and bifidobacteria (107 cfu.g -1 ) at the end of storage period (21 d).

Objectives: The beta-lactamases with extended spectrum of activity (ESBL) are medically one of the most important group of enzymes. Another group of beta-lactamases representing of Enterobacteriaceae is group of the AmpC-type cephalosporinases. The presented study provides identification and determination of the spectrum of resistance against different and clinically used antimicrobial drugs in the clinical isolates of Escherichia coli.Methods: These isolates had origin in different departments of the L. Pasteur University Hospital in Košice. The goal was the detection of betalactamase production with extended-spectrum effect and testing of AmpC-type cephalosporinases by several phenotypic tests in clinical isolates. MALDI-TOF MS analysis was performed on a Microflex MALDI Biotyper. Samples were positively tested for ESBL with the use of the disc diffusion method. PCR were performed with a series of primers designed for the detection of Ambler class A, B and C beta-lactamase genes.Results: For all 485 isolates, we determined the production of ESBL, which we detected in 166 E. coli isolates, which represents a 34.2% prevalence of ESBL production. It is clear from the results that the prevalence of ESBL-producing E. coli out of the total number of E. coli investigated reached 34.2%. In the monitored period, we confirmed at least one resistance gene from 485 E. coli in 188 positive isolates.Conclusions: We describe a complex ESBL epidemiology. The study revealed a high rate of ESBL-producing E. coli isolates; bla TEM and bla SHV enzymes dominated in ESBL-positive E. coli isolates in the L. Pasteur University Hospital in Košice.

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Abstract:The beta-lactamases with extended spectrum of activity (ESBL) are medically one of the most important group of enzymes. The presented study provides identification and determination of the spectrum of resistance against different and clinically used antimicrobial drugs in the clinical isolates of Escherichia coli. These isolates had their origin in different departments of the University Hospital L. Pasteur in Košice. The second goal was the detection of beta-lactamase production with extendedspectrum effect (ESBL) and testing of AmpC-type cephalosporinases by several phenotypic tests in clinical isolates. We used both the microdilution method and the method with an active agent, respectively. Samples were positively tested for ESBL with the use of the CLSI disk diffusion method. PCRs were performed with a series of primers designed for the detection of Ambler class A, B and C beta-lactamase genes. About 307 strains of E. coli were investigated. The growth of E. coli resistance to selected antibiotics was present in 83.25% of clinical isolates. There were identified 85 positive isolates in the studied group and the prevalence of the ESBL positive strains of E. coli reached 27.78%. An E. coli strain was isolated with mutations in the promoter region of the AmpC chromosomal gene that is associated with overproduction of the relevant enzyme. We describe a complex ESBL epidemiology. The study revealed a high rate of ESBL-producing E. coli isolates. The bla TEM and bla SHV enzymes dominated in ESBL-positive E. coli isolates in the University Hospital L. Pasteur in Košice.