Knowing spatial patterns of functional microbial guilds can increase our understanding of the relationships between microbial community ecology and ecosystem functions. Using geostatistical modeling to map spatial patterns, we explored the distribution of the community structure, size, and activity of one functional group in N cycling, the denitrifiers, in relation to 23 soil parameters over a 44-ha farm divided into one organic and one integrated crop production system. The denitrifiers were targeted by the nirS and nirK genes that encode the two mutually exclusive types of nitrite reductases, the cd 1 heme-type and copper reductases, respectively. The spatial pattern of the denitrification activity genes was reflected by the maps of the abundances of nir genes. For the community structure, only the maps of the nirS community were related to the activity. The activity was correlated with nitrate and dissolved organic nitrogen and carbon, whereas the gene pools for denitrification, in terms of size and composition, were influenced by the soil structure. For the nirS community, pH and soil nutrients were also important in shaping the community. The only unique parameter related to the nirK community was the soil Cu content. However, the spatial pattern of the nirK denitrifiers corresponded to the division of the farm into the two cropping systems. The different community patterns, together with the spatial distribution of the nirS/nirK abundance ratio, suggest habitat selection on the nirS-and nirK-type denitrifiers. Our findings constitute a first step in identifying niches for denitrifiers at scales relevant to land management.

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Characterization of spatial patterns of functional microbial communities could facilitate the understanding of the relationships between the ecology of microbial communities, the biogeochemical processes they perform and the corresponding ecosystem functions. Because of the important role the ammonia-oxidizing bacteria (AOB) and archaea (AOA) have in nitrogen cycling and nitrate leaching, we explored the spatial distribution of their activity, abundance and community composition across a 44-ha large farm divided into an organic and an integrated farming system. The spatial patterns were mapped by geostatistical modeling and correlations to soil properties and ecosystem functioning in terms of nitrate leaching were determined. All measured community components for both AOB and AOA exhibited spatial patterns at the hectare scale. The patchy patterns of community structures did not reflect the farming systems, but the AOB community was weakly related to differences in soil pH and moisture, whereas the AOA community to differences in soil pH and clay content. Soil properties related differently to the size of the communities, with soil organic carbon and total nitrogen correlating positively to AOB abundance, while clay content and pH showed a negative correlation to AOA abundance. Contrasting spatial patterns were observed for the abundance distributions of the two groups indicating that the AOB and AOA may occupy different niches in agro-ecosystems. In addition, the two communities correlated differently to community and ecosystem functions. Our results suggest that the AOA, not the AOB, were contributing to nitrate leaching at the site by providing substrate for the nitrite oxidizers.

In Scandinavia high losses of soil and particulate‐bound phosphorus (PP) have been shown to occur from tine‐cultivated and mouldboard‐ploughed soils in clay soil areas, especially in relatively warm, wet winters. The omission in the autumn of primary tillage (not ploughing) and the maintenance of a continuous crop cover are generally used to control soil erosion. In Norway, ploughing and shallow cultivation of sloping fields in spring instead of ploughing in autumn have been shown to reduce particle transport by up to 89% on highly erodible soils. Particle erosion from clay soils can be reduced by 79% by direct drilling in spring compared with autumn ploughing. Field experiments in Scandinavia with ploughless tillage of clay loams and clay soils compared to conventional autumn ploughing usually show reductions in total P losses of 10–80% by both surface and subsurface runoff (lateral movements to drains). However, the effects of not ploughing during the autumn on losses of dissolved reactive P (DRP) are frequently negative, since the DRP losses without ploughing compared to conventional ploughing have increased up to fourfold in field experiments. In addition, a comprehensive Norwegian field experiment at a site with high erosion risk has shown that the proportion of DRP compared to total P was twice as high in runoff water after direct drilling compared to ploughing. Therefore, erosion control measures should be further evaluated for fields with an erosion risk since reduction in PP losses may be low and DRP losses still high. Ploughless tillage systems have potential side‐effects, including an increased need for pesticides to control weeds [e.g. Elytrigia repens (L.) Desv. ex Nevski] and plant diseases (e.g. Fusarium spp.) harboured by crop residues on the soil surface. Overall, soil tillage systems should be appraised for their positive and negative environmental effects before they are widely used for all types of soil, management practice, climate and landscape.

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