A two-year-old cat from Tenerife, Canary Islands (Spain), presented with two months history of uveitis in the left eye. It had been treated for two months but still showed an active uveitis. After new treatment, eye examination showed a mobile worm in the anterior chamber. Following surgical removal, the worm was obtained. Morphological study revealed that it was a male metastrongyloid nematode (Nematoda: Metastrongyloidea) with caudal bursa and two similar spicules. Molecular tools based on the 18Sr RNA gene sequence identified the parasite as Gurltia paralysans, which is a neurotropic nematode previously found in South America. Therefore, this article describes the first report of ophthalmic case of parasitism by G. paralysans, and cites for first time this species out of South America.
Toll. The blood samples collected from 138 horses in each zone were centrifuged, stored at -20 • C, and later tested by competitive ELISA. A random sample of 71 positive horse sera was analyzed by Plaque Reduction Neutralization Test (PRNT) on Vero cells against African Horse Sickness Virus (AHSV) serotypes. Cytopathogenic effects were read four days later. Statistical data analysis was done using R software.Results: The overall true seroprevalence rate by ELISA test was 89.5% in 414 horse sera. In the subset of 71 infected horses, antibodies against AHSV 2 were detected in 67.5%, against AHSV 7 in 92.96% and against AHSV 9 in 100% of horses. AHSV 7 antibodies were found in 80.95% of the positive horses from Ross-Bethio, 95.45% from St.-Louis, and 100% in Richard-Toll zones.Conclusion: Prior to this study, only the monovalent vaccine against AHSV serotype 9 was used on horses in the area. The polyvalent vaccine against eight types of AHSV was first used after our sampling. Our results reveal the presence of antibodies against AHSV 7, which suggests that Serotype 7 virus had been circulating prior to the mass vaccination. Chi-square test for the three serotypes showed that they are not independent. Based on these results we hypothesize that the vectors of AHSV 9 in the Senegal River delta may be the same vectors that introduced and spread AHSV 2 and AHSV 7. This work is the first description of AHSV 7 in Senegal.http://dx. Purpose:The aim of this work was to increase the knowledge about the range of pathogens affecting domestic cats and dogs in the Canary Islands, Spain, due to the actual scarcity of data. Another purpose of this study was to assess through immunological methods whether dogs in the Canary Islands are coming into contact with the nematode Angiostrongylus cantonensis.Methods & Materials: Between 2014 and 2015, 264 dogs and 60 cats were attended in different veterinary clinics in Tenerife and Lanzarote, two out of the Canary Islands. Faeces samples were obtained from each individual to determine the presence of Cryptosporidium spp., Giardia spp., Toxoplasma spp. and Entamoeba spp. by morphological methods, whereas Giemsa-stained blood smears were occasionally analyzed for the presence of Babesia parasites. On the other hand, sera samples were obtained from 59 dogs belonging to Tenerife to carry out the detection of IgG antibodies against a 31-kDa glycoprotein of A. cantonensis by ELISA technique.Results: The protozoa Giardia spp., Cryptosporidum spp., Cystoisospora spp. and Entamoeba spp., and the helminths Toxocara canis, Trichuris vulpis, Enterobius sp., Aelurostrongylus abstrusus, Dicrocoelium sp., Toxascaris leonina and Ancylostoma caninun were found in domestic dogs. With regard to domestic cats, we found the protozoa Giardia spp., Cryptosporidum spp., Entamoeba spp. and Toxoplasma gondii, and the helminths Toxocara cati and Aelurostrongylus abstrusus. Giardia spp. was the most prevalent pathogen in both domestic cats and dogs. The presence of Cryptosporidium spp. in both cats and dogs was ocasi...
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