Selenium (Se) is one of the elements that determine the normal functioning of an organism; it has antioxidant properties and protects the organism against the actions of free radicals and carcinogenic factors. Selenium is an element that fulfils an important physiological function (Kieliszek and Blažejak 2013), but apart from all the elements, selenium has one of the narrowest range between dietary deficiency (< 40 µg/day) and toxic levels (> 400 µg/day) (Fordyce 2007).While there is no evidence of Se need for higher plants, several reports show that when Se added at low concentrations, it exerts beneficial effects on plant growth. Se may act as quasi-essential micronutrient through altering different physiological and biochemical traits; thus, plants vary considerably in their physiological and biochemical response to Se (El-Ramady et al. 2016).During the last decade, studies related with strategies for Se biofortification in food plants for human nutrition have increased significantly because this metalloid is incorporated into human metabolism mainly as a constituent of food plants (Mora et al. 2015). Biofortification is defined as the process of increasing the bioavailable concentrations of essential elements in the edible portions of cultivated plants through agricultural management (fertilization) or genetic improvement (White and Brodlay 2005).Plant-derived foodstuffs, namely cereals, are the major dietary sources of Se in most countries throughout the world, even if Se contents are strongly dependent upon the corresponding levels in cereal-growing soils. Therefore, wheat is one of the staple crops that appears as an obvious candidate for Se biofortification, considering its Possibility of selenium biofortification of winter wheat grain

The aim of the experiment was to study the importance of sulphur in oilseed rape (Brassica napus L.) nutrition as well as the effect of rising doses of sulphur in combination with nitrogen on yield, oiliness, oil production, nutrients content in seed and nutrients uptake by rapeseed. The plot–scale experiment was established in years 2013/14 and 2014/15 within the agricultural cooperative in Mojmírovce. There were four fertilization treatments on 600 m2 experimental plots in three replications in this experiment. The first treatment was unfertilized control. Other three treatments were fertilized by the same nitrogen dose of 160 kg.ha−1 and by increasing doses of sulphur. The second treatment was fertilized by a dose of 15 kg.ha−1 S, the third by a dose of 40 kg.ha−1 and a dose of 65 kg.ha−1 S was applied at the fourth treatment. The highest average yield 3.96 t.ha-1 was found when a dose of 40 kg.ha−1 S was applied. The application of sulphur in a dose of 65 kg.ha−1 was accompanied by a yield decrease by 11.4 % as compared to the treatment where a sulphur dose of 40 kg.ha−1 was used. An average oil content of 45.1, 45.5, and 44.0 % was found in treatments in which the doses of sulphur of 15, 40 and 65 kg.ha−1 were applied. No significant difference among the treatments fertilized by sulphur was found. The average oil production reached 1809, 1802 and 1595 kg.ha−1 in cases of treatments fertilized by sulphur doses of 15, 40 and 65 kg.ha−1.

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The aim of the experiment was to investigate the effect of nitrogen on oilseed rape (Brassica napus L.) yield as well as evaluate the level of nitrogen nutrition based on the value N. . Treatment 3 was fertilized three times during spring and treatment 2 was fertilized except 3 spring doses also in autumn. Results showed that yield of rapeseed was greatly influenced by weather conditions. Both experimental years were not equable in precipitation and temperatures. The highest average yield (at 12% moisture) 3.69 t * ha -1 was reached at treatment 3, where any autumn dose of nitrogen was not applied. It means an increase by 55.04% and 24.66% compared to unfertilized control treatment and treatment 2. Autumn dose of nitrogen did not influence significantly yield of seeds. Values of N.N.I. ranged from 0.71% to 1.25% and there were not observed any significant differences among treatments as well as between experimental years.